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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
CD64 Monoclonal Antibody (10.1), PE
- 宿主:
Mouse
- 供应商:
嵘崴达
- 保质期:
见产品外包装
- 库存:
需确认
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 亚型:
IgG1
- 规格:
500ul
CD64 Monoclonal Antibody (10.1), PE
货号 CD6404
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文献和实验Monoclonal Antibody Production
. (7) Centrifuge samples at 10,000g 10 min. (8) Pipet off the serum supernatant.Store at -20 ℃.This is your pre-bleed control. Hybridoma Fusion 2. For one fusion you will need (1) 8500 ml bottles of DMEM media (2) 3500 ml bottles of fetal calf serum (FCS
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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