Roche货号10108626001聚腺苷酸[别名:Poly

Roche货号10108626001聚腺苷酸[别名:Poly(A)]上海睿安生物19121878899默克Merck-Sigma-Millipore-Roche官方正式授权代理商

Roche货号10108626001，(规格100mg/瓶)╳目录单价¥3669.08元/瓶

Poly(A)

lyophilized,suitable for PCR,pkg of 100mg

别名:Poly(A),聚腺苷酸

属性

description:Polyadenylic acid

form:lyophilized

mol wt:700-3500 kDa

packaging:pkg of 100mg

manufacturer/tradename:Roche

concentration:0.5mg/ml(Working concentration)

technique(s):      PCR:suitable

color:white

solubility:      water:soluble

absorbance ratio :A_{290/260 nm} 0.03-0.05,A_{280/260 nm} 0.28-0.32,A_{250/260 nm} 0.86-0.90

storage temp:2-8°C

Quality Level:100

说明

General description:Poly(A)被用作DNA和RNA定量沉淀的载体。

Application:Poly(A)已可用于微滴式数字PCR(ddPCR)分析。

聚腺苷酸Poly(A)抑制Exo1的核酸外切酶活性。还用作重悬冻干gBlocks 的载体。

Biochem/physiol Actions

聚腺苷酸[Poly(A)]尾部有3′末端，在细胞核产生。哺乳动物细胞中，约含 250个核苷酸。Poly(A)可调控mRNA降解和翻译起始。胞质Poly(A)的延伸调控翻译过程。

Physical form:冻干品，钾盐

Preparation Note

工作浓度:0.5mg/ml        建议浓度为0.5mg/ml。
工作溶液:建议浓度为0.5mg/ml。

Analysis Note

典型分析:2.3μmol/mg Poly(A)(来自吸光度)相当于一个单核苷酸单位。色谱均匀。
吸光度测定A₂₅₀/A₂₆₀、A₂₈₀/A₂₆₀、A₂₉₀/A₂₆₀

Other Notes

1个A₂₆₀单位相当于40μg ssRNA。

仅用于生命科学研究。不可用于诊断。

链长为2.100至10.000个核苷酸

同行评审论文(部分:文献)

Poly(A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor

Kuhn U,et al.

The Journal of Biological Chemistry,284(34),22803-22814(2009)

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.

Yuichiro Miyaoka et al.

Scientific reports,6,23549-23549(2016-04-01)

Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that

Poly(ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities

Cheruiyot A,et al.

DNA Repair,35,106-115(2015)

Cells recognize osmotic stress through liquid-liquid phase separation lubricated with poly(ADP-ribose).

Kengo Watanabe et al.

Nature communications,12(1),1353-1353(2021-03-03)

Cells are under threat of osmotic perturbation; cell volume maintenance is critical in cerebral edema, inflammation and aging, in which prominent changes in intracellular or extracellular osmolality emerge. After osmotic stress-enforced cell swelling or shrinkage, the cells regulate intracellular osmolality

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Peiguo Yang et al.

Cell,181(2),325-345(2020-04-18)

The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in