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Roche货号10109878001胰蛋白酶抑制剂(别名:胰

蛋白酶)19121878899上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商
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  • ¥2568.77
  • Roche
  • 10109878001
  • made in USA
  • 2026年05月29日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 英文名

      Trypsin Inhibitor

    • 保质期

      1年

    • 保存条件

      2-8°C

    • 库存

      100⁺瓶

    • 供应商

      上海睿安生物19121878899

    • 规格

      1g/瓶

    Roche货号10109878001胰蛋白酶抑制剂(别名:胰蛋白酶)19121878899上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商

    胰蛋白酶抑制剂

    from chicken egg white

    别名:胰蛋白酶

    属性

    biological source:chicken egg white

    form:powder

    packaging:pkg of 1g

    manufacturer/tradename:Roche

    concentration:1mg/ml

    technique(s):      cell culture | mammalian:suitable

    optimum pH:3.8-4.5

    solubility:   water:soluble

    storage temp:2-8°C

    说明

    General description

    蛋清胰蛋白酶抑制剂是无血清细胞培养基中胰蛋白酶、纤溶酶和血浆激肽释放酶活性的抑制剂。它不抑制金属、半胱氨酸和天冬氨酸蛋白酶或组织激肽释放酶(丝氨酸蛋白酶)。

    Application

    胰蛋白酶抑制剂已用于:
    • 作为裂解液组分,用于处理Tsc2fl/fl(对照)和Tsc2fl/flLyz2-Cre(敲除[KO])骨髓来源巨噬细胞(BMDM),以便进行免疫印迹
    • 在脑室区细胞培养物中的干细胞完成消化解离后,灭活胰蛋白酶 
    • 在大脑组织完成解离和胰酶消化后,中和胰蛋白酶
    • 终止组织解聚和用于传代培养程序。
    鸡胚胰蛋白酶抑制剂用于终止组织解聚和传代培养程序。

    Preparation Note

    2至8°C下储存。(干态保存!)
    工作浓度: 0
    工作液: 推荐溶剂为蒸馏水。
    贮备液:1mg/ml
    储存条件(工作液):-15 至-25℃
    在蒸馏水或 1 mM hcl 中至少可稳定 6 个月,当以等分试样冷冻储存时。避免反复冻融循环。
    两者均溶于水。推荐的贮备液:1mg/mL。15°C 至-25°C 条件下等分冷冻储存。至少可稳定储存6个月。

    Other Notes:仅用于生命科学研究。不可用于诊断。

    同行评审论文(部分:文献)

    Membrane properties and electrogenesis in the distal axons of small dorsal root ganglion neurons in vitro.
    Vasylyev DV and Waxman SG
    Journal of Neurophysiology, 108(3), 729-740 (2012)
    Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions.
    Maksim Sinyuk et al.
    Journal of visualized experiments : JoVE, (160) (2020-06-23)
    The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine neonates for the isolation of glial subpopulations. The purpose of the procedure is to dissociate microglia, oligodendrocyte progenitor
    Production and use of lentivirus to selectively transduce primary oligodendrocyte precursor cells for in vitro myelination assays.
    Haley M Peckham et al.
    Journal of visualized experiments : JoVE, 95(95), 52179-52179 (2015-02-05)
    Myelination is a complex process that involves both neurons and the myelin forming glial cells, oligodendrocytes in the central nervous system (CNS) and Schwann cells in the peripheral nervous system (PNS). We use an in vitro myelination assay, an established
    Inhibition of Nonsense-Mediated Decay Induces Nociceptive Sensitization through Activation of the Integrated Stress Response.
    June Bryan de la Peña et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 43(16), 2921-2933 (2023-03-10)
    RNA stability is meticulously controlled. Here, we sought to determine whether an essential post-transcriptional regulatory mechanism plays a role in pain. Nonsense-mediated decay (NMD) safeguards against translation of mRNAs that harbor premature termination codons and controls the stability of ∼10%
    Numb regulates Tau levels and prevents neurodegeneration in tauopathy mouse models.
    Lacomme, et al.
    Science Advances, 8, eabm4295-eabm4295(2023)

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    图标文献和实验
    该产品被引用文献

    Roche货号10109878001胰蛋白酶抑制剂(别名:胰蛋白酶)19121878899上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商

    胰蛋白酶抑制剂

    from chicken egg white

    别名:胰蛋白酶

    属性

    biological source:chicken egg white

    form:powder

    packaging:pkg of 1g

    manufacturer/tradename:Roche

    concentration:1mg/ml

    technique(s):      cell culture | mammalian:suitable

    optimum pH:3.8-4.5

    solubility:   water:soluble

    storage temp:2-8°C

    说明

    General description

    蛋清胰蛋白酶抑制剂是无血清细胞培养基中胰蛋白酶、纤溶酶和血浆激肽释放酶活性的抑制剂。它不抑制金属、半胱氨酸和天冬氨酸蛋白酶或组织激肽释放酶(丝氨酸蛋白酶)。

    Application

    胰蛋白酶抑制剂已用于:
    • 作为裂解液组分,用于处理Tsc2fl/fl(对照)和Tsc2fl/flLyz2-Cre(敲除[KO])骨髓来源巨噬细胞(BMDM),以便进行免疫印迹
    • 在脑室区细胞培养物中的干细胞完成消化解离后,灭活胰蛋白酶 
    • 在大脑组织完成解离和胰酶消化后,中和胰蛋白酶
    • 终止组织解聚和用于传代培养程序。
    鸡胚胰蛋白酶抑制剂用于终止组织解聚和传代培养程序。

    Preparation Note

    2至8°C下储存。(干态保存!)
    工作浓度: 0
    工作液: 推荐溶剂为蒸馏水。
    贮备液:1mg/ml
    储存条件(工作液):-15 至-25℃
    在蒸馏水或 1 mM hcl 中至少可稳定 6 个月,当以等分试样冷冻储存时。避免反复冻融循环。
    两者均溶于水。推荐的贮备液:1mg/mL。15°C 至-25°C 条件下等分冷冻储存。至少可稳定储存6个月。

    Other Notes:仅用于生命科学研究。不可用于诊断。

    同行评审论文(部分:文献)

    Membrane properties and electrogenesis in the distal axons of small dorsal root ganglion neurons in vitro.
    Vasylyev DV and Waxman SG
    Journal of Neurophysiology, 108(3), 729-740 (2012)
    Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions.
    Maksim Sinyuk et al.
    Journal of visualized experiments : JoVE, (160) (2020-06-23)
    The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine neonates for the isolation of glial subpopulations. The purpose of the procedure is to dissociate microglia, oligodendrocyte progenitor
    Production and use of lentivirus to selectively transduce primary oligodendrocyte precursor cells for in vitro myelination assays.
    Haley M Peckham et al.
    Journal of visualized experiments : JoVE, 95(95), 52179-52179 (2015-02-05)
    Myelination is a complex process that involves both neurons and the myelin forming glial cells, oligodendrocytes in the central nervous system (CNS) and Schwann cells in the peripheral nervous system (PNS). We use an in vitro myelination assay, an established
    Inhibition of Nonsense-Mediated Decay Induces Nociceptive Sensitization through Activation of the Integrated Stress Response.
    June Bryan de la Peña et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 43(16), 2921-2933 (2023-03-10)
    RNA stability is meticulously controlled. Here, we sought to determine whether an essential post-transcriptional regulatory mechanism plays a role in pain. Nonsense-mediated decay (NMD) safeguards against translation of mRNAs that harbor premature termination codons and controls the stability of ∼10%
    Numb regulates Tau levels and prevents neurodegeneration in tauopathy mouse models.
    Lacomme, et al.
    Science Advances, 8, eabm4295-eabm4295(2023)

    引用文献图片1

    引用文献图片2

    引用文献图片3

    引用文献图片4

    引用文献图片5

    引用文献图片6引用文献图片7

    相关实验
    • 植物蛋白质组学和糖基化

      碳酸氢铵,pH 8.0。 ( 2 ) 胰蛋白酶:来源于牛胰腺的经 TPCK 处理的胰蛋白酶Sigma  Aldrich,  Fluka 产品)( 保存于 -20°C ) 。 ( 3 ) 胰凝乳蛋白酶: 来源于牛胰腺的经 TLCK 处理的胰凝乳蛋白酶(Sigma Aldrich) ( 保存于 -20°C ) 。 ( 4 ) C18 色谱柱:Merck C18 Spherisorb ODS 2 色谱柱(5 μm,4.6 mm X 250 mm,VWR)。 ( 5 ) 溶液

    • 常用生化试剂

      B1281    Trypsin Inhibitor, (胰蛋白酶抑制剂) 100mg 140 Amresco分装 B1282 Tryptone(胰蛋白胨) 500g 380 Oxoid原装 B1283 酵母粉 500

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    文献支持
    Roche货号10109878001胰蛋白酶抑制剂(别名:胰蛋白酶)19121878899上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商
    ¥2568.77