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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Suc-LLVY-AMC(Proteasome Substrate)
- 供应商:
武汉艾美捷
- 规格:
5mg
产品名称:Suc-LLVY-AMC,蛋白酶体底物Suc-LLVY-AMC(Proteasome Substrate)
产品货号:LSS-PS500
产品规格:5mg
产品描述:用于测量20S蛋白酶体,钙蛋白酶和其他胰凝乳蛋白酶样蛋白酶的胰凝乳蛋白酶样肽酶活性的荧光底物(Ex/Em=380nm/460nm)。
保存建议:Suc-LLVY-AMC,蛋白酶体底物建议收到产品后置于-80℃中保存。
背景资料:UPS(Ubiquitin-proteasome system,泛素-蛋白酶体系统)是细胞内蛋白质降解的多组分系统,它参与细胞的生长、分化,DNA复制与修复,细胞代谢、免疫反应等重要生理生化过程。蛋白酶体(2.5MDa):由2个19S和1个20S亚单位组成的桶状结构,19S为调节亚单位,位于桶状结构的两端,识别多聚泛素化蛋白并使其去折叠。19S亚单位上还具有一种去泛素化的同功肽酶,使底物去泛素化。20S为催化亚单位,位于两个19S亚单位的中间,其活性部位处于桶状结构的内表面,可避免细胞环境的影响。
点击:Suc-LLVY-AMC,蛋白酶体底物Suc-LLVY-AMC(Proteasome Substrate)查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看每日生物评论网站。
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文献和实验Studies of the Ubiquitin Proteasome System
that is regulated at the level of both addition and removal of ubiquitin from target proteins. This unit includes a number of different basic protocols that will facilitate the study of components of the ubiquitin system and substrate ubiquitylation
Homogeneous, Bioluminescent Proteasome Assays
, bioluminescent assays for all three proteasome activities, the chymotrypsin-like, trypsin-like, and caspase-like, using purified proteasome. We have also applied this technology to a cellular assay using the substrate for the chymotrypsin-like activity
Assays for Proteasome Inhibition
The ubiquitin-proteasome pathway has an essential role in the regulation of numerous cellular proteins, including those mediating inflammatory conditions and cancer (1 –6 ). Intracellular proteins destined for proteolysis are first tagged
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