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- 文献和实验
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- 库存:
4448
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
4543T/96T
| 规格: | 4543T | 产品价格: | ¥4447.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥3650.0 |
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
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