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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-17643R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
SPCS3
- 抗体名:
信号肽复合物亚基3抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Cow, Horse,
- 免疫原:
KLH conjugated synthetic peptide derived from human SPCS3:51-120/180
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | SPCS3 |
| 中文名称 | 信号肽复合物亚基3抗体 |
| 别 名 | DKFZp564J1864; FLJ22649; Microsomal signal peptidase 22/23 kDa subunit; Microsomal signal peptidase 23 kDa subunit; PRO3567; Signal peptidase complex subunit 3; Signal peptidase complex subunit 3 homolog (S. cerevisiae); Signal peptidase complex subunit 3 homolog; SPase 22 kDa subunit; SPase 22/23 kDa subunit; SPC22; SPC22/23; SPC3; UNQ1841; YLR066W. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Horse, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 20kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPCS3:51-120/180 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: SPCS3 (signal peptidase complex subunit 3), also known as SPC3, is a 180 amino acid single-pass type II membrane protein that localizes to both the microsome and the endoplasmic reticulum (ER) and belongs to the SPCS (signal peptidase complex subunit) family. Existing as a component of the microsomal signal peptidase complex which consists of five members, SPCS3 functions to remove signal peptides from proteins that are translated to the lumen of the ER. The gene encoding SPCS3 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: A component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Subunit: Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3. Subcellular Location: Endoplasmic reticulum and Microsome membrane;: Single-pass type II membrane protein Similarity: Belongs to the SPCS3 family. SWISS: P61009 Gene ID: 60559 Database links: Entrez Gene: 767917 Cow Entrez Gene: 404005 Dog Entrez Gene: 60559 Human Entrez Gene: 76219 Mouse Entrez Gene: 76976 Mouse Entrez Gene: 100360464 Rat Entrez Gene: 317409 Rat SwissProt: Q3SZU5 Cow SwissProt: P61008 Dog SwissProt: P61009 Human SwissProt: Q9D365 Mouse SwissProt: Q568Z4 Rat Unigene: 6322 Cow Unigene: 3875 Dog Unigene: 42194 Human Unigene: 328305 Mouse Unigene: 482965 Mouse Unigene: 162344 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPCS3) Polyclonal Antibody, Unconjugated (bs-17643R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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文献和实验一、ELISA的原理 ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性,酶标记的抗原或抗体既保留其免疫学活性,又保留酶的活性。在测定时,受检标本(测定其中的抗体或抗原)与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成的抗原抗体复合物与液体中的其他物质分开。再加入酶标记的抗原或抗体,也通过反应而结合在固相载体上。此时固相上的酶量与标本中受检物质的量呈一定的比例。加入酶反应的底物后,底物被酶催化成为有色产物,产物的量与标本中
质和细胞膜,而且还有许多膜性结构的细胞器,在细胞须内合成的蛋白质怎样的到达细胞的不同部位呢?了解比较清楚的是分泌性蛋白质的转运。 像原核细胞一要,真核细胞合成的蛋白质N端也有信号肽也能形成两个α螺旋的发夹结构,这个结构可插入到内质网的膜中,将正在合成中的多肽链带和内质网内腔。80年代中期在胞浆中发现一种由小分子RNA和蛋白质共同组成的复合物,它能特异地与信号肽识别而命名为信号肽识别颗粒。它的作用是识别信号肽与核糖体结合并暂时阻断多肽链的合成。内质网外膜上的SRP受体,当ARP与受体结合后,信号肽
一、ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性,酶标记的抗原或抗体既保留其免疫学活性,又保留酶的活性。在测定时,受检标本(测定其中的抗体或抗原)与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成的抗原抗体复合物与液体中的其他物质分开。再加入酶标记的抗原或抗体,也通过反应而结合在固相载体上。此时固相上的酶量与标本中受检物质的量呈一定的比例。加入酶反应的底物后,底物被酶催化成为有色产物,产物的量与标本中受检
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