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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0478 |
---|---|
产品名称 | Human OSM/Oncostatin M ELISA Kit |
产品描述 | For quantitative detection of human OSM in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, A26R-220 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | OSM |
基因全名 | oncostatin M |
Uniprot ID | P13725 |
指标别名 | MGC20461|ONCM_HUMAN|Oncostatin M precursor|Oncostatin-M|OncostatinM|OSM|OTTMUSP00000005249|RP23-453B22.1 |
背景资料 | OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0478-CAP | 96-well plate precoated with anti- human OSM antibody | 1 |
EK0478-ST | Lyophilized recombinant human OSM standard | 10ng/tubex2 |
EK0478-DA | Biotinylated anti- human OSM antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 159 | 453 | 36 | 158 | 453 |
Standard deviation | 1.96 | 11.6 | 29.44 | 2.19 | 13.27 | 36.69 |
CV(%) | 4.9% | 7.3% | 6.5% | 6.1% | 8.4% | 8.1% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.023 | 0.113 | 0.210 | 0.332 | 0.589 | 1.062 | 1.574 | 2.251 |
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