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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1625 |
---|---|
产品名称 | Human GSTA1 ELISA Kit |
产品描述 | For quantitative detection of human GSTA in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,A2-F222 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | GSTA1 |
基因全名 | glutathione S-transferase alpha 1 |
Uniprot ID | GSTA1: P08263 |
指标别名 | Glutathione S-transferase A2; GST HA subunit 2; GST class-alpha member 2; GST-gamma; GSTA2-2; GTH2; GSTA2; GST2 |
背景资料 | GSTA encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1625-CAP | 96-well plate precoated with anti- human GSTA antibody | 1 |
EK1625-ST | Lyophilized recombinant human GSTA standard | 10ng/tubex2 |
EK1625-DA | Biotinylated anti- human GSTA antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 298 | 1176 | 6136 | 305 | 1197 | 6246 |
Standard deviation | 13.1 | 55.27 | 331.3 | 19.22 | 80.2 | 462.2 |
CV(%) | 4.4% | 4.7% | 5.4% | 6.3% | 6.7% | 7.4% |
Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.065 | 0.188 | 0.295 | 0.598 | 1.039 | 1.333 | 1.716 | 1.916 |
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