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RNF14 antibody

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  • ¥1380 - 2200
  • 康朗生物
  • 中国/美国/德国
  • 2026年02月28日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200
  • Rabbit
  • Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
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    • 详细信息
    • 技术资料
    • 供应商

      上海康朗生物科技有限公司

    • 库存

      大量

    • 目录编号

      kl-9225R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      12个月

    • 抗体英文名

      RNF14 antibody

    • 抗体名

      环指蛋白14抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,

    • 免疫原

      KLH conjugated synthetic peptide derived from human RNF14:71-170/474

    • 亚型

      IgG

    • 形态

      冻干粉或液体

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200

    • 浓度

      1mg/ml

    • 保存条件

      -20 °C

    • 规格

      100ul 200ul

    RNF14 antibody
    中文名称 环指蛋白14抗体
    别    名 Androgen receptor associated protein 54; Androgen receptor-associated protein 54; ARA 54; ARA54; E3 ubiquitin protein ligase RNF14; E3 ubiquitin-protein ligase RNF14; FLJ26004; HFB 30; HFB30; HRIHFB2038; RING finger protein 14; RNF 14; Rnf14; RNF14_HUMAN; TRIAD 2; TRIAD2; Triad2 protein.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  信号转导  表观遗传学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 54kDa
    细胞定位 细胞核 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human RNF14:71-170/474 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.

    Function:
    Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. 

    Subunit:
    Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity.

    Subcellular Location:
    Cytoplasm. Nucleus.

    Tissue Specificity:
    Widely expressed.

    Post-translational modifications:
    RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination. [SIMILARITY] 

    Similarity:
    Belongs to the RBR family. RNF14 subfamily. 
    Contains 1 IBR-type zinc finger. 
    Contains 2 RING-type zinc fingers. 
    Contains 1 RWD domain. 

    SWISS:
    Q9UBS8

    Gene ID:
    9604

    Database links:

    Entrez Gene: 9604 Human

    Entrez Gene: 56736 Mouse

    Entrez Gene: 619577 Rat

    Omim: 605675 Human

    SwissProt: Q9UBS8 Human

    SwissProt: Q9JI90 Mouse

    Unigene: 483616 Human

    Unigene: 228903 Mouse

    Unigene: 115357 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample: Cerebellum (Mouse) Lysate at 40 ug
    Primary: Anti-RNF14 (bs-9225R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54 kD
    Observed band size: 50 kD

    产品细节图片2
    Sample: Placenta (Mouse) Lysate at 40 ug
    Primary: Anti-RNF14 (bs-9225R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54 kD
    Observed band size: 50 kD

    产品细节图片3
    Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAM160B1) Polyclonal Antibody, Unconjugated (bs-9225R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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