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COLUMN REACTOR COMPLETE 6.3 ML
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文献和实验Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
and larger cartridges, where the volume of the lysate is sufficient to effect a complete hydration and wash of the filter paper. To get the maximal recovery of DNA, rinse the filter paper with an additional 1.5 ml of water. Equilibration:
the protein sample.MALDI targetMicrocentrifugeMicrocentrifuge tubes (standard and 0.7-mL)METHODFigure 1. Schematic of affinity binding of phosphopeptides to immobilized metal ion affinity columns.Preparation of the Immobilized Metal Ion Affinity Column Insert
(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
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