手机验证
上海联迈生物工程有限公司
大量
LM-1495R
多克隆
Rabbit
1年
OCLN
紧密连接蛋白抗体
Rabbit
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig,
KLH conjugated synthetic peptide derived from mouse OCLN:431-522/522 <Cytoplasmic>
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=3µg/Test (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
50ul 100ul 200ul
英文名称 | OCLN |
中文名称 | 紧密连接蛋白抗体 |
别 名 | Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin. |
Specific References (6) | bs-1495R has been referenced in 6 publications. [IF=2.46] Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926. Pig. PubMed:24473141 [IF=2.73] Sun, Yan‐Na, et al. "Effects of insulin combined with idebenone on blood–brain barrier permeability in diabetic rats." Journal of Neuroscience Research (2014). WB ; Rat. PubMed:25421718 [IF=2.71] Guo, Xiaoling, et al. "Matrigel and Activin A promote cell-cell contact and anti-apoptotic activity in cultured human retinal pigment epithelium cells." Experimental Eye Research (2016). IF(ICC) ; Human. PubMed:27130547 [IF=2.86] Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512. WB, IHC-P ; Pig. PubMed: 22272087 [IF=2.46] Pan, Li, et al. "Effects of Soybean Agglutinin on Mechanical Barrier Function and Tight Junction Protein Expression in Intestinal Epithelial Cells from Piglets." International Journal of Molecular Sciences 14.11 (2013): 21689-21704. Pig. PubMed:24189218 [IF=2.03] Gu, Yan-ting, et al. "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." Neuroscience letters 493.3 (2011): 140-144. WB ; Rat. PubMed:21334421 |
|
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 细胞生物 免疫学 转录调节因子 细胞粘附分子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=3µg/Test (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 57kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from mouse OCLN:431-522/522 <Cytoplasmic> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]. Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Subunit: Interacts with TJP1/ZO1 and with VAPA. Subcellular Location: Membrane; Multi-pass membrane protein. Cell junction, tight junction. Tissue Specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts. Similarity: Belongs to the ELL/occludin family. Contains 1 MARVEL domain. SWISS: Q61146 Gene ID: 18260 Database links: Entrez Gene: 18260 Mouse Entrez Gene: 100506658 Human Entrez Gene: 397236 Pig Entrez Gene: 83497 Rat Entrez Gene: 403844 Dog Omim: 602876 Human SwissProt: Q28269 Dog SwissProt: Q16625 Human SwissProt: Q61146 Mouse SwissProt: Q6P6T5 Rat Unigene: 592605 Human Unigene: 4807 Mouse Unigene: 31429 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Sample: A549 Cell Lysate at 30 ug Primary: Anti- OCLN (bs-1495R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-OCLN (bs-1495R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample: A549 Cell Lysate at 30 ug Primary: Anti- OCLN (bs-1495R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 60 kD The blue histogram is unstained cells (293FT cells). The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody. The green histogram is cells stained with Rabbit Anti-OCLN antibody (bs-1495R) plus secondary antibody (Concentraion 1: 20). |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。