Anti-Human VEGF 165 (Polyclona

产品描述：

Source:Polyclonal Rabbit

Preparation:Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF 165 matrix.

Immunogen:E.coli derived Recombinant Human VEGF₁₆₅ (PeproTech catalog# 100-20)

Sandwich ELISA:To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human VEGF (500-P10Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.

Western Blot:To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


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