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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 库存:
需确认
- 英文名:
Mouse VEGF-165 Recombinant Protein, PeproTech®
- 规格:
2ug
产品描述
VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant Murine VEGF 165 is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
Source:E.coli
Synonyms:Vascular Endothelial Growth Factor, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF-A
AA Sequence (monomer):MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity:≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml.
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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
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Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Abstract Table of Contents Materials Figures Literature Cited Abstract Protein microarrays
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