Recombinant Mouse B7-H3

Sulfyhydryl-Reactive Site-Directed Cross-Linking as a Method for Probing the Tetrameric Structure of Histones H3 and H4

of both recombinant and native chromatin complexes consisting either of histone subunits alone or in association with accessory proteins, in this case histone chaperones. The approaches described may be generally applicable for monitoring the interactions of a diverse

Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes

with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles

Purification of Recombinant Poly(ADP-Ribose) Polymerases

, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.