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- 详细信息
- 文献和实验
- 技术资料
- CAS号:
见产品说明书
- 英文名:
ALBUMIN BOVINE FRACTION V,
- 保质期:
见产品说明书
- 保存条件:
见产品说明书
- 库存:
大量
- 规格:
1 KG
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文献和实验Purification of a VesicleVacuole (V) Fraction from Aspergillus
and stress response, it is important to understand the composition and function of the membrane-bound organelles that house this biosynthetic machinery. Isolation of vesicles, endosomes, and vacuoles (V fraction) is, therefore, an essential method to study
Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Purification of Calmodulin from Bovine Brain
. Procedure (0-4℃): 1.About 1 kg of bovine brain (PelFreeze,frozen)is homogenized in 2 volumes of homogenizing buffer (50 mM MOPS,pH 7.0,1.0 mM 2-ME,1.0 mM EDTA)for 90 sec in a large Waring blender. 2.The homogenate is centrifuged at 10,000 x g for 1 hr
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