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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-14615R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Eps8L3
- 抗体名:
EPS8相关蛋白3抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
- 免疫原:
KLH conjugated Synthesised phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr317:1-100/593
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | Eps8L3 |
| 中文名称 | EPS8相关蛋白3抗体 |
| 别 名 | EPS8 like protein 3; Epidermal growth factor receptor kinase substrate 8 like protein; Epidermal growth factor receptor kinase substrate 8 like protein 3; Epidermal growth factor receptor pathway substrate 8 related protein 3; EPS8 like 3; EPS8 like protein 3; EPS8 related protein 3; EPS8R3. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Sheep, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr317:1-100/593 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008] Function: EPS8 like protein 3 is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. Alternatively spliced transcript variants encoding different isoforms exist. Subunit: Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Interacts with FASLG. Subcellular Location: Cytoplasmic Tissue Specificity: Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments. SWISS: Q8TE67 Gene ID: 79574 Database links: Entrez Gene: 79574 Human SwissProt: Q8TE67 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Eps8L3) Polyclonal Antibody, Unconjugated (bs-14615R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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文献和实验金酸(HAuCl·Cl3 ·4H2 O) 2. 葡萄球菌A蛋白(SPA) 3. 细胞毒素相关蛋白A(CagA) 4. 酶联免疫吸附试验(ELISA)检测抗CagA-IgG试剂盒 实验设备 1. Z 323 K低温高速离心机 2. LGJ 0.5-Ⅱ冷冻干燥机 3. 点膜器 实验材料 血清标本 实验步骤
摘 要: 目的 建立一种快速、简易的检测血清中抗幽门螺杆菌(Hp)细胞毒素相关蛋白A(CagA)抗体的胶体金免疫层析法(GICA)。 方法 采用柠檬酸三钠还原法制备胶体金颗粒,标记葡萄球菌A蛋白(SPA),将重组的CagA抗原划线固定于硝酸纤维素膜上,制成免疫层析检测试条。 血清中IgG与测试条上金标记物结合后沿着硝酸纤维素膜移动,与膜上的固相抗体结合形成肉眼可见的红色线条。 结果 用GICA与ELISA试剂盒对比检测
重大突破!马光辉/魏炜/李玉华团队 Nature 子刊报道新型精准治疗性白血病疫苗
Biomedical Engineering研究内容作者的前期临床研究结果表明,EPS8 和 PD-1/PD-L1 在白血病患者中高表达,可以分别用作新型白血病抗原和白血病疫苗的检查点靶标。 图片来源:Nature Biomedical Engineering由于会被快速清除,用 EPS8 抗原肽和 PD-1 抗体(免疫检查点抑制剂)混合直接接种的效果并不好。因此,作者利用自愈合大孔微球构建了疫苗递送新剂型,其新颖的「后包埋」策略实现了抗原肽和抗体的高效装载,并充分保护了二者的活性。这种自愈合大孔微球
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