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- Thermo
- 16600082
- 美国
- 2025年10月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
99
- 保质期：
已收到实际货物为准
- 供应商：
北京诺博莱德科技有限公司
- 保存条件：
已收到实际货物为准
货号： 16600082
规格
| Form: | Liquid |
|---|---|
| Volume: | 500 ml |
| Glutamine: | L-Glutamine |
| HEPES Buffer: | No HEPES |
| Product Size: | 500 mL |
| Phenol Red Indicator: | Phenol Red |
| Sodium Pyruvate Additive: | No Sodium Pyruvate |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
描述
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Bacto-peptone | |
| • Phenol Red |
The complete formulation is available.
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hank's salts to enable use outside a CO2 incubator.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® McCoy's 5A (modified) is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® McCoy's 5A (modified) product made in our Scotland facility (26600-023). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco® McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco® McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
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文献和实验的(小牛血清)RPMI培养基 4. 肝素钠钠盐(HEPES，最低99.5％，Sigma -Aldrich公司) 5. Ⅱ型胶原酶 6. 皂素(Sigma公司，＃S-7900) 7. BSA(牛血清白蛋白) 8. Azida(2mM) 实验设备 1. 剪刀 2. 钳子 3. 培养皿 4. 1.5，2.0，15和50毫升管 5. 热孵化器（振荡
肝素蛋白多糖(HSPG)。前三种蛋白的摩尔含量一般差不多相等，HSPG的含量则偏低，但是鉴于ECM Gel是生物提取物，我们不能确定各成分的准确浓度。 ECM Gel来源于细胞裂解物还是真正的细胞基质？ ECM Gel基质胶由整个EHS肿瘤（不是分离细胞）制备而成。它是肿瘤的全提取产物。 ECM基质胶与Matrigel®(Corning)、Geltrex®(Thermo)或Cultrex®(Trevigen)一样吗？ ECM Gel基质胶是这些产品的生物等效品，ECM基质胶（E6909，E1270）由默克
mM的HEPES。 你查一下HEPES的分子量，就知道怎么配了。低血清培养基，就是培养基里面加入的血清比较少，eg：我要配0.1%血清的培养基，就是100ml的培养基加入0.1ml的血清。 24、流式细胞的问题： 问：细胞用一抗包被后，然后再和二抗结合，不知道这样能否用流式细胞检测？由于我要检测的蛋白在国内外没有可用于流式细胞检测的现货，有个公司虽然可制备，但需要6个月，并且价格一个蛋白要5万元以上，时间和经费均不足，只好想想其它办法，不知道这样可否？ 答：当然可以先用自己的一抗，再用荧光标记的二抗
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