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- Thermo
- 11765054
- 美国
- 2025年10月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 保质期：
已收到实际货物为准
- 供应商：
北京诺博莱德科技有限公司
- 保存条件：
已收到实际货物为准
货号： 11765054
规格
| Form: | Liquid |
|---|---|
| Volume: | 500 ml |
| Glutamine: | L-Glutamine |
| HEPES Buffer: | No HEPES |
| Product Size: | 500 mL |
| Phenol Red Indicator: | Phenol Red |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
描述
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.
Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
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文献和实验的(小牛血清)RPMI培养基 4. 肝素钠钠盐(HEPES，最低99.5％，Sigma -Aldrich公司) 5. Ⅱ型胶原酶 6. 皂素(Sigma公司，＃S-7900) 7. BSA(牛血清白蛋白) 8. Azida(2mM) 实验设备 1. 剪刀 2. 钳子 3. 培养皿 4. 1.5，2.0，15和50毫升管 5. 热孵化器（振荡
基质（CC131），稀释比例为1:80。 根据所需的体积按相应比例上调或下调。 以下是用1.5 mL 1:80稀释的ECM凝胶包被6孔板的示例。 首先进行1:20稀释，操作步骤是，在15 mL锥形管中，将0.5 mL ECM凝胶加入9.5 mL冷的DMEM / F12或DMEM培养基中。 总体积 = 10 mL. 需要进一步稀释4倍才能稀释成最终的1:80。 将2.5 mL 1:20稀释的ECM凝胶加入7.5 mL冷的DMEM / F12培养基中。 总体积 = 10 mL 注意：建议的稀释度为1:80
mM的HEPES。 你查一下HEPES的分子量，就知道怎么配了。低血清培养基，就是培养基里面加入的血清比较少，eg：我要配0.1%血清的培养基，就是100ml的培养基加入0.1ml的血清。 24、流式细胞的问题： 问：细胞用一抗包被后，然后再和二抗结合，不知道这样能否用流式细胞检测？由于我要检测的蛋白在国内外没有可用于流式细胞检测的现货，有个公司虽然可制备，但需要6个月，并且价格一个蛋白要5万元以上，时间和经费均不足，只好想想其它办法，不知道这样可否？ 答：当然可以先用自己的一抗，再用荧光标记的二抗
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