These lyophilised calibrators from Chromsystems are based on human serum and should be used according to the instruction manual. They are designed to calibrate the measurement method in clinical diagnostics for the quantitative determination of 25-OH-Vitamin D3 and 25-OH-Vitamin D2 in serum/plasma. While following the instructions the calibrators should be handled and measured in the same manner as a patient specimen. These calibrators are only available as multilevel calibrator set with 6 different concentration levels plus 1 blank value.
TECHNICAL DATA
Method of Analysis
LC-MS/MS
Content
7 x 1 ml (lyoph.)
Parameter
25-OH-vitamin D2, 25-OH-vitamin D3
Concentrations
Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly or downloaded from our website after login.
Substance
Unit
Calibrator 1
Calibrator 2
Calibrator 3
Calibrator 4
Calibrator 5
Calibrator 6
Blank Calibrator
25-OH-Vitamin D3
µg/l nmol/l
9.1 22.6
19.0 47.5
33.8 84.2
66.0 165
105 261
141 353
n.d.
25-OH-Vitamin D2
µg/l nmol/l
4.7 11.4
14.0 33.9
28.5 69.1
56.1 136
102 247
144 348
n.d.
n.d. = not detected (< LOQ)
Reconstitution
Carefully remove the metal seal and rubber stopper from the vials. Add exactly 1.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.
Storage conditions
Stored below -18 °C and unopened, the lyophilised calibrators are stable until the expiry date specified on the vial labels. The reconstituted calibrator can be stored in up to 1 weeks tightly capped at +2 to +8 C. If the calibrators cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.