- ¥1580
- 康朗生物
- kl-7451R
- 中国/美国/德国
- 2025年11月20日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- Rabbit
- Human, Rat,
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- 详细信息
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-7451R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
Histone H3 (acetyl K27) antibody
- 抗体名：
乙酰化组蛋白H3抗体
- 宿主：
Rabbit
- 适应物种：
Human, Rat,
- 免疫原：
KLH conjugated Synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K27:AR(Ac-K)SA
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
Histone H3 (acetyl K27) antibody
| 中文名称 | 乙酰化组蛋白H3抗体 |
| 别 名 | acetyl H3; Histone H3(acetyl k27); H3K27; H 3; H3 3 Like Sequence MH921; H3 3A; H3; H3 Histone; H3 Histone Family Member E Pseudogene; H3.4; H3/A; H3/g; H31_HUMAN; H3F3; H3FA; H3FT; H3t; HIST1H3J; HIST3H3; Histone cluster 1, H3a; Histone H3 3 Pseudogene; Histone H3.1; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 乙酰化抗体 |
| 研究领域 | 肿瘤 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K27:AR(Ac-K)SA |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription. DISEASE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Similarity: Belongs to the histone H3 family. SWISS: P68431 Gene ID: 8290 Database links: Entrez Gene: 8290 Human Entrez Gene: 8350 Human Entrez Gene: 8351 Human Entrez Gene: 8352 Human Entrez Gene: 8353 Human Entrez Gene: 8354 Human Entrez Gene: 8355 Human Entrez Gene: 8356 Human Entrez Gene: 8357 Human Entrez Gene: 8358 Human Entrez Gene: 8968 Human Entrez Gene: 260423 Mouse Entrez Gene: 319148 Mouse Entrez Gene: 319149 Mouse Entrez Gene: 319150 Mouse Entrez Gene: 319151 Mouse Entrez Gene: 319152 Mouse Entrez Gene: 319153 Mouse Entrez Gene: 360198 Mouse Entrez Gene: 97908 Mouse Entrez Gene: 100364501 Rat Entrez Gene: 100365669 Rat Entrez Gene: 291159 Rat Entrez Gene: 314977 Rat Entrez Gene: 364716 Rat Entrez Gene: 679950 Rat Entrez Gene: 679994 Rat Entrez Gene: 680511 Rat Entrez Gene: 680599 Rat Entrez Gene: 682330 Rat Entrez Gene: 691496 Rat Omim: 601128 Human Omim: 602810 Human Omim: 602811 Human Omim: 602812 Human Omim: 602813 Human Omim: 602814 Human Omim: 602815 Human Omim: 602816 Human Omim: 602817 Human Omim: 602818 Human Omim: 602819 Human SwissProt: P68431 Human SwissProt: P84243 Human SwissProt: Q16695 Human SwissProt: Q6NXT2 Human SwissProt: Q71DI3 Human SwissProt: P68433 Mouse SwissProt: P84228 Mouse SwissProt: Q6LED0 Rat Unigene: 132854 Human Unigene: 247813 Human Unigene: 247814 Human Unigene: 248176 Human Unigene: 443021 Human Unigene: 484990 Human Unigene: 532144 Human Unigene: 533292 Human Unigene: 546315 Human Unigene: 586261 Human Unigene: 591778 Human Unigene: 221301 Mouse Unigene: 261657 Mouse Unigene: 377874 Mouse Unigene: 390558 Mouse Unigene: 397328 Mouse Unigene: 138090 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (acetyl K27)) Polyclonal Antibody, Unconjugated (bs-7451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (acetyl K27)) Polyclonal Antibody, Unconjugated (bs-7451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): B16(purple). Primary Antibody (green line): Rabbit Anti-Histone H3 (acetyl K27)antibody (bs-4316R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 20 min on ice.The cells were then incubated in 10% goat serum to block non-specific protein-protein interactions for 30min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Histone H3 (acetyl K27) antibody
¥1580
