2′-脱氧鸟苷5′-三磷酸 三钠盐
dGTP
Lithium salt
别名: 2′-deoxy-guanosine-5′-triphosphate, dGTP-Na3, deoxy-guanosine-5′-triphosphate, 2′-
General description
dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.
Application
2′-脱氧鸟苷5′-三磷酸三钠盐可用于DNA测序、PCR和其他基于DNA聚合酶的DNA合成和修复技术。
Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
Biochem/physiol Actions
2′-脱氧鸟苷5′-三磷酸可用于体内合成,通过DNA聚合酶合成DNA。
Quality
Typical analysis:
• ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
• Absorbance determination A250/A260, A280/A260, A290/A260
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR
Unit Definition
1 μmol dGTP = 13.7 A252 units
11051466001 |
2'-Deoxy-guanosine-5'-TP Solut |
250 µl (25 µmol) |
11051482001 |
dTTP, 25 Ámol |
250 µl (25 µmol) |
11420470001 |
dUTP, 25 Ámol |
250 µl (25 µmol) |
11585622910 |
Fluorescein High Prime 100ul |
100 µl (25 reactions) |
11922505001 |
Desoxy-NTP Set, slt. |
40 x 10 µmol (40 x 100 µl) |
11585541001 |
PCR Core Kit^PLUS |
1 kit (for 50 reactions of 50 µl final reaction volume) |
5061369001 |
FuGENE HD Transfection Reagent |
10 ml (up to 3,000 transfections) |