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- 详细信息
- 文献和实验
- 技术资料
- 库存:
需确认
- 保存条件:
−20°C
- 保质期:
见产品外包装
- 供应商:
嵘崴达
- 英文名:
2'-Deoxy-guanosine-5'-TP Solut
- 规格:
250 μL
2′-脱氧鸟苷5′-三磷酸 三钠盐
dGTP
Lithium salt
别名: 2′-deoxy-guanosine-5′-triphosphate, dGTP-Na3, deoxy-guanosine-5′-triphosphate, 2′-
General description
dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.
Application
2′-脱氧鸟苷5′-三磷酸三钠盐可用于DNA测序、PCR和其他基于DNA聚合酶的DNA合成和修复技术。
Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
Biochem/physiol Actions
2′-脱氧鸟苷5′-三磷酸可用于体内合成,通过DNA聚合酶合成DNA。
Quality
Typical analysis:
• ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
• Absorbance determination A250/A260, A280/A260, A290/A260
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR
Unit Definition
1 μmol dGTP = 13.7 A252 units
| 11051466001 | 2'-Deoxy-guanosine-5'-TP Solut | 250 µl (25 µmol) |
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| 11585541001 | PCR Core Kit^PLUS | 1 kit (for 50 reactions of 50 µl final reaction volume) |
| 5061369001 | FuGENE HD Transfection Reagent | 10 ml (up to 3,000 transfections) |
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文献和实验Single Primer ("Semi-Random") P
Po: F1284 (TP89) 38 bp from end; will not work in TPOPs; CAAGATGTGTATCTACCTTAAC Po or Pn: IS10R2 (TP134) 27 bp from end; right side of TPOPs only; CAAGATGTGTATCCACCTTAACTTAATGATTTT Pn: IS10R4 (TP134) 4 bp from end; any Tn10 derivative
Cell cycle analysis of Escherichia coli cells
: The C+D period is estimated from the initiation age (ai ), the generation time (τ) and the number of generations spanned per cell cycle. Example: 4-origin-cells: 23 % Generation time (τ): 27 min Initiation age (ai ): 5 min
项目 单位 X±SD 样本数 参考
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