WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
75kDa
细胞定位
细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human Vinexin:401-500/671
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: Vinexin is a 671 amino acid protein that is expressed as two isoforms, designated Vinexin alpha and Vinexin beta. Localized to cell junctions in both the cytoplasm and the cytoskeleton, Vinexin alpha functions to promote Actin stress fiber formation, playing an important role in modification of the Actin cytoskeleton. Like Vinexin alpha, Vinexin beta is localized to cell junctions in the cytoplasm, but is also found in the nucleus where it plays an important role in cell spreading and in activation of the JNK pathway in response to EGF stimulation. Although Vinexin alpha and Vinexin beta have different roles within the cell, both proteins contain three SH3 domains in their carboxy terminus and are expressed in a variety of tissues, including placenta, heart, liver, brain, pancreas and skeletal muscle. Together, Vinexin alpha and Vinexin beta are involved in cell-cell adhesion, signal transduction and cytoskeletal organization throughout the cell.
Function: Vinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
Subcellular Location: Localized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
SWISS: O60504
Gene ID: 10174
Database links:
Entrez Gene: 10174 Human
Omim: 610795 Human
SwissProt: O60504 Human
Unigene: 528572 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.