- ¥1380 - 2200
- LMAI Bio
- LM-12863R
- 进口/国产
- 2025年07月14日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Zebrafish, Sheep, Xenopus laevis
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-12863R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
TUBB5
- 抗体名：
微管蛋白β5抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Zebrafish, Sheep, Xenopus laevis
- 免疫原：
KLH conjugated synthetic peptide derived from human TUBB5:301-444/444
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | TUBB5 |
| 中文名称 | 微管蛋白β5抗体 |
| 别 名 | beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; TBB5_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 产品类型 | 内参抗体 |
| 研究领域 | 细胞类型标志物 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Zebrafish, Sheep, Xenopus laevis |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TUBB5:301-444/444 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Subunit: Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1. Subcellular Location: Cytoplasmic, cytoskeleton. Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). Similarity: Belongs to the tubulin family. SWISS: P07437 Gene ID: 203068 Database links: Entrez Gene: 203068 Human Entrez Gene: 22154 Mouse Entrez Gene: 29214 Rat Omim: 191130 Human SwissProt: P07437 Human SwissProt: P99024 Mouse SwissProt: P69897 Rat Unigene: 636480 Human Unigene: 2458 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Eye (mouse) Lysate at 40 ug Primary: Anti- TUBB5 (bs-12863R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kD  Sample: Bone (mouse) Lysate at 40 ug Primary: Anti- TUBB5 (bs-12863R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD  Sample: hela (human)cell Lysate at 40 ug Primary: Anti- TUBB5(bs-12863R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBB5) Polyclonal Antibody, Unconjugated (bs-12863R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验而导致不孕。接着对这五名患者及家系中的其他成员，加上另外 23 个患病家系的 DNA 样本，利用 sanger 测序检测候选基因 TUBB8 的突变情况。然后利用 RT-PCR 技术检测卵母细胞、早期胚胎、精子细胞和部分组织中的 TUBB8 和其他 β-微管蛋白亚型的表达。 α-微管蛋白多肽和 β-微管蛋白多肽组装形成异二聚体，研究人员利用实验评估 TUBB8 突变是否影响体外异二聚体组装、HeLa 细胞系中的微管结构、酵母细胞中的微管动力学以及小鼠和人类卵母细胞的纺锤体组装
人类生殖繁衍主要依赖于成熟卵细胞和精子融合形成的受精卵，但是阻滞人卵细胞成熟的遗传机制至今未知。 研究人员利用外显子组测序技术对不孕症患者四代家系中的五名成员进行研究，其中三名患者因卵母细胞第一次减数分裂受到抑制而导致不孕。接着对这五名患者及家系中的其他成员，加上另外 23 个患病家系的 DNA 样本，利用 sanger 测序检测候选基因 TUBB8 的突变情况。然后利用 RT-PCR 技术检测卵母细胞、早期胚胎、精子细胞和部分组织中的 TUBB8 和其他 β-微管蛋白
内参就是内部参照，一般是指管家基因编码表达的蛋白，它们在各个组织和细胞中的表达相对恒定，在检测蛋白的表达水平变化时常用它来做参照参。内参则可以校正蛋白质定量、上样过程中存在的误差，保证实验结果的准确性。 此外，内参可以作为空白对照，还可以检测蛋白转膜情况是否完全、整个 WB 显色发光体系是否正常。内参抗体种类很多，比如 β-Actin、β-Tubulin、GAPDH 等，下面简单介绍下如何选择内参。 一、目的蛋白分子量 选择内参抗体时，应该考虑目的蛋白分子量的大小。通常应该保证目的
技术资料暂无技术资料 索取技术资料
