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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-5054R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
CYP4A1 antibody
- 抗体名:
细胞色素P450 4A1抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human CYP4A1:351-450/519
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:100-500
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
50ul 100ul 200ul
| 中文名称 | 细胞色素P450 4A1抗体 |
| 别 名 | 20-HETE synthase; 20-hydroxyeicosatetraenoic acid synthase; CP4AB_HUMAN; Cyp4a1; Cyp4a10; CYP4A11; Cyp4a14; Cyp4a3; CYP4A7; CYP4AII; CYPIVA11; Cytochrome P-450HK-omega; Cytochrome P450 4A1; Cytochrome P450 4A10; Cytochrome P450 4A11; Cytochrome P450 4A14; Cytochrome P450 4A2; Cytochrome P450 4A3; Cytochrome P450 4A7; Cytochrome P450HL-omega; Fatty acid omega-hydroxylase; Lauric acid omega-hydroxylase. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 脂蛋白 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 59kDa |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP4A1:351-450/519 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. [provided by RefSeq, Jul 2008]. Function: Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE). Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Tissue Specificity: Kidney and liver. Similarity: Belongs to the cytochrome P450 family. SWISS: Q02928 Gene ID: 1579 Database links: Entrez Gene: 1579 Human Entrez Gene: 24306 Rat Entrez Gene: 298423 Rat Entrez Gene: 50549 Rat Omim: 601310 Human SwissProt: Q02928 Human SwissProt: P14581 Rabbit SwissProt: P08516 Rat SwissProt: P20816 Rat SwissProt: P20817 Rat Unigene: 1645 Human Unigene: 726474 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: 293T (Human) Lysate at 40 ug Primary: Anti-CYP4A1 (bs-5054R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD |
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文献和实验Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families
at an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This method is applicable to enzymatic studies to determine the catalytic activity of cDNA-expressed human enzymes in the CYP1, CYP2, and CYP3 families, and 7-ethoxycoumarin O -deethylation
Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families
in the human CYP1, CYP2, and CYP3 families are active catalysts of 7-ethoxycoumarin O-deethylation (3 ). Consequently, the 7-ethoxycoumarin O-deethylase assay has been used to verify the catalytic activity of a panel of recombinant human P450s in the CYPl, CYP
PLoS Genet. 2009 March; 5 (3) : e1000433. Published online 2009 March 20. doi: 10.1371/journal.pgen.1000433 A Genome-Wide Association Study Confirms VKORC1 , CYP2C9 , and CYP4F2 as Principal Genetic
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