Tissue factor/CD142 antibody

价格¥780 - 2200

品牌康朗生物
货号kl-4690R
产地中国/美国/德国
更新日期2025年11月27日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500
宿主Rabbit
适应物种Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,

上海康朗生物科技有限公司

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详细信息
技术资料

供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-4690R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
Tissue factor/CD142 antibody
抗体名：
组织因子抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
免疫原：
KLH conjugated synthetic peptide derived from human Tissue factor:32-100/295 <Extracellular>
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500
浓度：
1mg/ml
保存条件：
-20 °C
规格：
50ul 100ul 200ul

Tissue factor/CD142 antibody

中文名称	组织因子抗体
别名	CD 142; CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor; TF_HUMAN.
	Specific References (3) \| bs-4690R has been referenced in 3 publications. [IF=7.13] Gleeson, Birgitta M., et al. "Bone marrow‐derived mesenchymal stem cells have innate procoagulant activity and cause microvascular obstruction following intracoronary delivery: Amelioration by anti‐thrombin therapy." STEM CELLS (2015). Pig. PubMed:25969127 [IF=2.98] Milano, M., et al. "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." Journal of Applied Toxicology 36.1 (2016): 151-160 WB ; Rat. PubMed:25858758 [IF=22.39] Fletcher, Russell B., et al. "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." Cell Stem Cell (2017). FCM ; Mouse. PubMed:28506465
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	细胞生物生长因子和激素细胞表面分子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	29kDa
细胞定位	细胞膜分泌型蛋白
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Tissue factor:32-100/295 <Extracellular>
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Subunit: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Subcellular Location: Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Lung, placenta and pancreas. Similarity: Belongs to the tissue factor family. SWISS: P13726 Gene ID: 2152 Database links: Entrez Gene: 2152 Human Omim: 134390 Human SwissProt: P13726 Human Unigene: 62192 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Polyclonal Antibody, Unconjugated (bs-4690R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated(bs-4690R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control black line): A431 (balck). Primary Antibody (green line): Rabbit Anti-Tissue factor CD142/AF647 antibody (bs-4690R-AF647) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-AF647 . Protocol The cells were fixed with 4% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 20 min on ice. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed. Blank control black line): A431 (balck). Primary Antibody (green line): Rabbit Anti-Tissue factor CD142/AF488 antibody (bs-4690R-AF488) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-AF488 . Protocol The cells were fixed with 4% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 20 min on ice. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.