- ¥780 - 2200
- LMAI Bio
- LM-1225R
- 进口/国产
- 2025年07月14日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-1225R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Adenosine A3 Receptor
- 抗体名：
腺苷受体A3抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 免疫原：
KLH conjugated synthetic peptide derived from human ADORA3:85-180/347
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | Adenosine A3 Receptor |
| 中文名称 | 腺苷受体A3抗体 |
| 别 名 | adenosine A3 receptor; A3AR; AD 026; AD026; ADORA 3; ADORA3; bA552M11.5; RP11 552M11.7; TGPCR1; adenosine receptor A3 isoform 1; adenosine receptor A3 isoform 2; AA3R_HUMAN. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 心血管 神经生物学 细胞膜受体 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADORA3:85-180/347 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. Subcellular Location: Cell membrane. Post-translational modifications: Phosphorylation on Ser-317 may be crucial for rapid desensitization. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P33765 Gene ID: 140 Database links: Entrez Gene: 403805 Dog Entrez Gene: 140 Human Entrez Gene: 606753 Pig Omim: 600445 Human SwissProt: Q28309 Dog SwissProt: P33765 Human SwissProt: Q6P2N6 Human Unigene: 281342 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ADORA-3(腺苷受体A3)属于G蛋白偶联受体家族，在腺苷调节细胞活力和生长中起关键作用，并且在很多肿瘤中表达增高，A3AR可能成为肿瘤诊断标志物和治疗靶点。 |
| 产品图片 |  Sample: Colon carcinoma lysates (Human) Lysate at 40 ug Gastric carcinoma lysates (Human) Lysate at 40 ug Primary: Anti- ADORA3 (bs-1225R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 35 kD Observed band size: 35 kD  Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated(bs-1225R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-1225R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃). Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody(bs-1225R); Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test. |
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文献和实验Characterization of P1 (Adenosine) Purinoceptors
. 251:888‐893. Kim, Y.C., Ji, X.D., and Jacobson, K.A. 1996. Derivatives of the triazoloquinazoline adenosine antagonist (CGS 15943) are selective for the human A3 receptor
zhubaoqinzhu 各位大侠，我急需要搞清楚怎么选择小鼠基因组甲基化抗体！！！！！希望哪位专家给指点一下。非常感谢！ freecell 主要取决于你做什么试验，以后请别问这么莫名其妙的问题！ http://www.abgent.com/products/category/protein_modification/methylation zhubaoqinzhu 不好意思，我最近
luozhifeng339 请教各位达达，我准备做EMSA，如果做SUPER-SHIFT的话，该选择原装抗体还是分装的抗体呢，盼不吝赐教！ wanglinling 看到一篇EMSA的文章，不知道里面有没有回答你的问题哈，不过英文的值得阅读哈。 GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure
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