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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-12153R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
TXLNB
- 抗体名:
β-taxilin蛋白抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human TXLNB:331-430/684
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | TXLNB |
| 中文名称 | β-taxilin蛋白抗体 |
| 别 名 | Beta-taxilin; C6orf198; dJ522B19.2; DKFZp451A175; hMDP77; LST001; MDP77; Muscle-derived protein 77; RP3-522B19.2; Txlnb; TXLNB_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TXLNB:331-430/684 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Beta-taxilin promotes nerve regeneration and may be involved in intracellular vesicle transport. Expressed predominantly in cardiac and skeletal muscle, beta-taxilin binds to the coiled coil region of the syntaxin family members STX1A, STX3A, and STX4A. Beta- and gamma-taxilins, bind to the alpha subunit of the nascent polypeptide-associated complex (NAC) and affect its nuclear distribution, suggesting that the taxilin family is involved not only in the translational process through its interaction with NAC but also in the transcriptional process through its interaction with alphaNAC alone. Function: Promotes motor nerve regeneration (By similarity). May be involved in intracellular vesicle traffic. Subunit: Binds to the C-terminal coiled coil region of syntaxin family members STX1A, STX3A and STX4A. Has a preference for STX1A. Tissue Specificity: Expressed in skeletal muscle. Similarity: Belongs to the taxilin family. SWISS: Q8N3L3 Gene ID: 167838 Database links: Entrez Gene: 167838 Human Entrez Gene: 378431 Mouse Entrez Gene: 308622 Rat Omim: 611438 Human SwissProt: Q8N3L3 Human SwissProt: Q8VBT1 Mouse Unigene: 535820 Human Unigene: 283493 Mouse Unigene: 165855 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-TXLNB (bs-12153R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 76 kD Observed band size: 76 kD ![]() Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TXLNB) Polyclonal Antibody, Unconjugated (bs-12153R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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文献和实验Blot往往省略参照,导致结果出现问题时无法分析结果,即便有结果也可能影响结果的分析。 内参即是内部参照(Internal Control),对于哺乳动物细胞表达来说一般是指由管家基因编码表达的蛋白(Housekeeping Proteins),它们在各组织和细胞中的表达相对恒定,在检测蛋白的表达水平变化时常用它来做参照物。在western blotting实验中,除了需要进行蛋白抽提、蛋白定量、等量蛋白上样电泳、转膜、靶蛋白抗体孵育、显色等步骤以外,还需要进行内参的检测,以校正蛋白质定量
物。在Western Blotting 实验中,除了需要进行蛋白抽提、蛋白定量、等量蛋白上样电泳、转膜、靶蛋白抗体孵育、显色等步骤以外,还需要进行内参的检测,以校正蛋白质定量、上样过程中存在的实验误差,保证实验结果的准确性。 实际上内参是最容易被忽略的一项。我们知道,要用Western Blot比较不同条件下或者不同组织中,目的蛋白表达量的相对多少,前提条件是等量的细胞上样,才有比较的基础,特别表达量不高时,上样量的差别就很可能影响结果的分析,所以需要内参。在国外发表的文章中,Western
水平变化时常用它来做参照物。在Western Blotting 实验中,除了需要进行蛋白抽提、蛋白定量、等量蛋白上样电泳、转膜、靶蛋白抗体孵育、显色等步骤以外,还需要进行内参的检测,以校正蛋白质定量、上样过程中存在的实验误差,保证实验结果的准确性。在Western Blotting中使用内参其实就是在WB过程中的另外用内参对应的抗体检测内参,这样在检测目的产物的同时可以检测内参的表达,由于内参在各组织和细胞中的表达相对恒定,借助检测每个样品内参的量就可以用于校正上样误差,这样半定量的结果才更为可信
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