ID1 antibody

价格¥1380 - 2200

品牌康朗生物
货号
kl-3529R
产地中国/美国/德国
更新日期2025年12月30日

应用范围ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:200-800
宿主Rabbit
适应物种Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,

上海康朗生物科技有限公司

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详细信息
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供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-3529R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
ID1 antibody
抗体名：
DNA结合抑制因子1抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
免疫原：
KLH conjugated synthetic peptide derived from human ID1:51-155/155
亚型：
IgG
形态：
冻干粉或液体
应用范围：
ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:200-800
浓度：
1mg/ml
保存条件：
-20 °C
规格：
100ul 200ul

ID1 antibody

中文名称	DNA结合抑制因子1抗体
别名	bHLHb24; Class B basic helix-loop-helix protein 24; DNA binding protein inhibitor ID 1; DNA binding protein inhibitor ID1; DNA-binding protein inhibitor ID-1; Dominant negative helix loop helix protein; ID 1; ID; ID1; ID1_HUMAN; Inhibitor of Differentiation 1; Inhibitor of DNA binding 1.
规格价格	100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	100ul 200ul
研究领域	肿瘤细胞生物染色质和核信号信号转导转录调节因子表观遗传学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
产品应用	ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:200-800 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	16kDa
细胞定位	细胞核细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human ID1:51-155/155
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene. Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. Subunit: Heterodimer with other HLH proteins. Interacts with COPS5. Subcellular Location: Cytoplasm. Nucleus. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P41134 Gene ID: 3397 Database links: Entrez Gene: 3397 Human Entrez Gene: 15901 Mouse Entrez Gene: 25261 Rat Omim: 600349 Human SwissProt: P41134 Human SwissProt: P20067 Mouse SwissProt: P41135 Rat Unigene: 504609 Human Unigene: 444 Mouse Unigene: 2113 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-ID1 Antibody, conjugated (bs-3529R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-ID1 Antibody, conjugated (bs-3529R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Blank control: mouse spleen cells(blue). Primary Antibody: Rabbit Anti- ID1/FITC Conjugated antibody (bs-3529R /FITC, green line), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. Protocol The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-3529R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed. Blank control: mouse kidney cells(blue). Primary Antibody: Rabbit Anti- ID1/FITC Conjugated antibody (bs-3529R /FITC, green line), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. Protocol The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-3529R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed. Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-ID1 antibody (bs-3529R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.