ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
17kDa
细胞定位
分泌型蛋白
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
Recombinant human IL-1B (117-269):
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Subunit: Monomer.
Subcellular Location: Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Similarity: Belongs to the IL-1 family.
SWISS: P01584
Gene ID: 3553
Database links:
Entrez Gene: 3553 Human
Entrez Gene: 16176 Mouse
Entrez Gene: 24494 Rat
Omim: 147720 Human
SwissProt: P01584 Human
SwissProt: P10749 Mouse
SwissProt: Q63264 Rat
Unigene: 126256 Human
Unigene: 222830 Mouse
Unigene: 9869 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
生长因子和激素( Growth Factor and Hormones) IL-1β是一种糖蛋白,它既可诱导急性期时相关蛋白的产生,也是β细胞终末分化并分泌抗体的必需因子,在免疫调节中发挥重要做 用。 IL-1β前体在细胞内合成后被酶剪切为成熟形态后排出到细胞外,IL-1β的前体有153个氨基酸。成熟形态有116个氨基酸。