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- 详细信息
- 文献和实验
- 技术资料
- 库存:
280
- 英文名:
Recombinant Human R-spondin-1
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冻干蛋白置于-20℃以下可长期保存,室温
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博专业生产供应重组人Rspo1(R-spondin-1)(Wnt信号通路激活剂) 重组蛋白,我公司销*(代"售")全品类的分子生物学试剂,价格优惠,欢迎广大科研工作者垂询订购。
名称:重组人Rspo1(R-spondin-1)(Wnt信号通路激活剂) 重组蛋白
编号:JN1160
规格:10μg|50μg|500μg|1mg
英文名:Recombinant Human R-spondin-1
品牌:百奥莱博
产地:北京
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Arg31-Ala263在C端含有His标签。
RSPO1质量控制:>95%(还原性SDS-PAGE)
RSPO1制剂:冻干品
RSPO1保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
RSPO1复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于RSPO1:
RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accomp*(代"a")nies chemotherapy and radiation in cancer patients with head and neck tumors.
欲了解更多重组人Rspo1(R-spondin-1)(Wnt信号通路激活剂) 重组蛋白的品牌、产地、价格及说明书等产品信息,请致电北京百奥莱博科技有限公司,我们将竭诚为您服务,另外,以下产品正在火爆促销:
BTN120408 绿豆核酸酶 Mung Bean Nuclease
KFS002 柠檬酸*抗原修复液(50X)
SV1565 胰蛋白酶-ultra,质谱级
YT313 总抗氧化能力检测试剂盒(ABTS法) Total Antioxidant Capacity Assay Kit with ABTS method
BTN120698 青霉素G*盐溶液 Penicillin G Sodium Solution
ALH195 一步法荧光定量RT-PCR试剂盒 One Step qRT-PCR Kit
SV0851 Q5 热启动超保真 2X Master Mix
WE0302 定影粉 Fixing Powder
BTN70804 一步法单菌落质粒DNA提取试剂盒 One-step single colony plasmid DNA extraction Kit
SV1327 蓝色预染蛋白 Standard,宽范围 (11–190 kDa)
RFT169 c-Myc标签抗体 Anti-c-Myc tag antibody
KFS246 TRITC标记链霉亲和素 Rhodamine (TRITC)-conjugated Streptavidin
SY0486 体内巨噬细胞清除剂(中性**松) Clophosome-Clodronate Liposomes (Neutral)
YT622 彩色预染蛋白分子量标准(15-120kD) Prestained Color Protein Marker
BTN131032 人基因组DNA(女性) Human Genomic DNA(Female)
HR0411 细胞周期与细胞凋亡检测试剂盒 Cell cycle and apoptosis detection kit
重组人Rspo1(R-spondin-1)(Wnt信号通路激活剂) 重组蛋白关键词:重组人Wnt信号通路激活剂,重组人Rspo1蛋白,JN1160,重组人R-spondin-1
·重组人CLEC10A
编号:JN1853
英文名称:Recombinant Human C-Type Lectin Domain Family 10 Member A
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Gln61-His316在C端含有His标签。
CLEC10A质量控制:>95%(还原性SDS-PAGE)
CLEC10A制剂:冻干品
CLEC10A保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
CLEC10A复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于CLEC10A:
C-Type Lectin Domain Family 10 Member A (CLEC10A) is a type II transmembrane C-type lectin that is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. CLEC10A/MGL binds and internalizes molecules with terminal nonsialylated GalNAc carbohydrates such as the Tn carcinoma antigen. CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity. It constitute a unique class of C-type lectins because of their specificity for galactose and its structural homologues. CLEC10A is thought to participate in the recognition of molecules from both altered self and pathogens due to its monosaccharide specificity for Gal and N-acetylgalactosamine (GalNAc). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes.
重组人Rspo1(R-spondin-1)(Wnt信号通路激活剂) 重组蛋白关键词:重组人Wnt信号通路激活剂,重组人Rspo1蛋白,JN1160,重组人R-spondin-1
WE0198 动物组织/细胞RNA提取试剂盒(含DNase I) RNAtiqu Tissue&Cell RNA Extraction Kit(DNase I)
BTN3191 Tris饱和酚 Tris-Saturated Phenol
SV1052 三磷酸腺苷双磷酸酶 Apyrase
RFT018 DNA Marker I(100~600bp)
KFS143 微丝绿色荧光探针 Actin-Tracker Green
SY0372 8%预制胶,12孔 Precast Protein Gels, 8%, 12 wells
SV0172 BseRI限制性内切酶 BseRI Restriction Endonuclease
BTN81212B 一站式蛋白质非变性PAGE电泳套装(中pH) One-Stop Protein Native PAGE Pack(B)
HR0225 昆虫蛋白快速提取试剂盒(离心柱法,2D电泳用) Rapid extraction of insect protein Kit (centrifugal column method, 2D electrophoresis)
SY0020 定点突变试剂盒 Site-Directed Mutagenesis Kit
BTN120410 核酸外切酶I Exonulease I
BTN130907 脱脂奶粉 Non-fat Milk
SV1557 Furin 蛋白酶
YT308 抗氧化酶SOD(超氧化物歧化酶) Superoxide dismutase
KFS387 超氧化物歧化酶(SOD)测试盒(总SOD,黄嘌呤氧化酶)
我公司正在打折促销重组蛋白全系列产品,恭候您的咨询选购重组人Rspo1(R-spondin-1)(Wnt信号通路激活剂) 重组蛋白。
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文献和实验v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
the Wnt pathway for cancer therapeutics. Nat. Rev. Drug Discov. 5 , 997–1014. 6. Coudreuse, D. and Korswagen, H. C. (2007) The making of Wnt: new insights into Wnt maturation, sorting and secretion. Development 134 , 3–12. 7. Miller J. R. (2002
范围,该例为0.1-1.0 mg/ml。这个浓度范围对于不同的细胞因子或重组蛋白是不同的。如重组人FGF-6(产品编号:100-30)为0.5-1.0mg/ml,而重组人Activin B(产品编号:120-15)则一定要是0.5mg/ml。 高于或低于该浓度范围会有什么问题呢? 答:首先,细胞因子或蛋白会不稳定,即很容易出现活性下降的现象。其次,高于这个浓度范围,可能会超过该蛋白的最大溶解浓度,即蛋白无法完全溶解。再者,高于或低于该浓度范围,蛋白可能会出现聚集(aggregation
一、融合表达蛋白的纯化:融合表达蛋白可以在原目标分子之外带有GST肽段或(His)6肽段,从而使得可以分别用Glutathione Sepharose凝胶或Chelating Sepharose凝胶进行亲和色谱分子,一步可以达到~90%的纯度,经过特异蛋白酶切后,再进行离子交换及高分辨凝胶过滤一般便可以达到所需的纯度(95~99%)。二、包含体表达蛋白的纯化:在E.coli系统表达重组蛋白,表达量高时,常形成包含体,包含体易于与细胞其他组分分离,但需要注意的是蛋白复性的步骤。一般采用盐酸胍溶解
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