WB=1:500-2000 ELISA=1:500-1000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
31kDa
细胞定位
细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human CD335(131-200aa):198-206/304 <Extracellular>
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. NCR1 is a glycoprotein that has two extracellular Ig-like domains followed by a ~40 amino acid residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. NCR1 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. NCR1 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines.
Function: Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Subunit: Interacts with CD247 and FCER1G
Subcellular Location: Cell membrane.
Tissue Specificity: Selectively expressed by both resting and activated NK cells.
Similarity: Belongs to the natural cytotoxicity receptor (NCR) family. Contains 2 Ig-like (immunoglobulin-like) domains.
SWISS: O76036
Gene ID: 9437
Database links:
Entrez Gene: 9437 Human
Entrez Gene: 17086 Mouse
Entrez Gene: 117547 Rat
Omim: 604530 Human
SwissProt: O76036 Human
SwissProt: Q8C567 Mouse
SwissProt: Q9Z0H5 Rat
Unigene: 97084 Human
Unigene: 240231 Mouse
Unigene: 30049 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample:Raji (Human)Cell Lysate at 40 ug Primary: Anti-NCR1(bs-10027R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 36kD