CD147细胞外基质金属蛋白酶诱导因子抗体

价格¥780 - 2200

品牌LMAI Bio
货号LM-0684R
产地进口/国产
更新日期2026年06月05日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig,

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供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-0684R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
CD147
抗体名：
细胞外基质金属蛋白酶诱导因子抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig,
免疫原：
KLH conjugated synthetic peptide derived from human TCSF:301-385/38 <Cytoplasmic>
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
50ul 100ul 200ul

CD147细胞外基质金属蛋白酶诱导因子抗体

英文名称	CD147
中文名称	细胞外基质金属蛋白酶诱导因子抗体
别名	TCSF; Emmprin; 5A11 antigen; 5F7; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; BSG; CD 147; CD147 antigen; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; M6 leukocyte activation antigen; neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; BASI_HUMAN.
	Specific References (2) \| bs-0684R has been referenced in 2 publications. [IF=2.19] Cui, K., et al. "Transplantation of Autologous Bone Marrow Mononuclear Cells Regulates Inflammation in a Rabbit Model of Carotid Artery Atherosclerosis." Journal of Vascular Research 53.3-4 (2016): 196-205. WB ; Rabbit. PubMed:27788500 [IF=3.12] Tsai, Wen-Chiuan, et al. "EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas." Journal of Neuro-Oncology (2013): 1-10. Human. PubMed:23817808
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	肿瘤免疫学合成与降解细胞表面分子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	40kDa
细胞定位	细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human TCSF:301-385/38 <Cytoplasmic>
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008 Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Subunit: Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms heterooligomers of isoform 2 and isoform 3. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Melanosome. Tissue Specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Post-translational modifications: N-glycosylated. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P35613 Gene ID: 682 Database links: Entrez Gene: 682 Human Entrez Gene: 12215 Mouse Entrez Gene: 25246 Rat Omim: 109480 Human SwissProt: P35613 Human SwissProt: P18572 Mouse SwissProt: P26453 Rat Unigene: 501293 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation）主要由肿瘤细胞合成，刺激纤维母细胞产生MMP
产品图片	Sample: Liver(Mouse) Lysate at 40 ug Placenta(Mouse) Lysate at 40 ug HepG2 Cell Lysate at 40 ug Primary: Anti-CD147 (bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 60 kD Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (bs-0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (bs-0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD147/TCSF/Emmprin Polyclonal Antibody, Unconjugated(bs-0684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining lank control (blue line): MCF7 (blue). Primary Antibody (green line):Rabbit Anti-CD147 antibody(bs-0684R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.