WB=1:500-2000 ELISA=1:500-1000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
48kDa
细胞定位
细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human Nogo R:331-430/473
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: Axons are essential for neuronal communication but they do not regenerate after injury to the adult mammalian brain or spinal cord. Failed regeneration is due in part to the production of a potent axonal growth inhibitor, Nogo, by myelinating cells. The finding of a high affinity axonal receptor for the extracellular domain of Nogo provides the first insight into the basis of Nogo action. Disrupting the interaction of Nogo with the Nogo-66 receptor may facilitate axonal regeneration in vivo. The protein is dubbed the Nogo receptor because it binds with several other proteins that block neural growth. It is found to be ubiquitous in the brain and spinal cord.
Function: Receptor for RTN4, OMG and MAG. Mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. Acts in conjunction with RTN4 and LIGO1 in regulating neuronal precursor cell motility during cortical development (By similarity).
Subunit: Homomultimer. Interacts with LINGO1. Interacts with KIAA0319L.
Similarity: Belongs to the Nogo receptor family. Contains 8 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
SWISS: Q9BZR6
Gene ID: 65078
Database links:
Entrez Gene: 65078 Human
Entrez Gene: 65079 Mouse
Entrez Gene: 113912 Rat
Omim: 605566 Human
SwissProt: Q9BZR6 Human
SwissProt: Q99PI8 Mouse
SwissProt: Q99M75 Rat
Unigene: 30868 Human
Unigene: 40149 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample:Cerebrum (Mouse)Lysate at 40 ug Primary: Anti-Nogo R(bs-20486R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 48kD Observed band size: 48kD
Sample:Heart(Mouse)Lysate at 40 ug Primary: Anti-Nogo R(bs-20486R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 48kD Observed band size: 48kD