大家都在搜
手机验证
上海联迈生物工程有限公司
大量
LM-0547R
多克隆
Rabbit
1年
Endostatin
内皮抑素/内皮他丁抗体
Rabbit
Human, Mouse, Rat,
KLH conjugated synthetic peptide derived from human Endostatin:1581-1680/1754
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
50ul 100ul 200ul
英文名称 | Endostatin |
中文名称 | 内皮抑素/内皮他丁抗体 |
别 名 | Collagen XV alpha 1; Alpha 1 collagen type 18 (XVIII)(COL18A1); Alpha 1 type XVIII collagen; COL18A1; Collagen alpha 1(XVIII) chain; Collagen type XVIII alpha 1; FLJ27325; KNO; MGC74745; COL18A1; COIA1_HUMAN; Antiangiogenic agent; COIA1_HUMAN; COL15A1; COL18A1; Collagen alpha 1(XV) chain; Collagen alpha-1(XV) chain; Collagen type XV proteoglycan; Collagen type XVIII alpha 1; Collagen XV, alpha 1 polypeptide; Collagen, type XV, alpha 1; Endostatin; Endostatin XV; FLJ34914; FLJ38566; KNO1; KS; Multi functional protein MFP; OTTHUMP00000021782; OTTHUMP00000115472; OTTHUMP00000115473. |
Specific References (3) | bs-0547R has been referenced in 3 publications. [IF=0.00] Hu, Ming‑Ming, et al. "Clinical significance of serum and tumor tissue endostatin evaluation in operable non‑small cell lung cancer." Biomedical Reports. IHC-P ; Human. PubMed:25279166 [IF=4.10] Imoto, Keisuke, et al. "Endostatin is protective against monocrotaline-induced right heart disease through the inhibition of T-type Ca2+ channel."Pflügers Archiv-European Journal of Physiology (2016): 1-12. IHC-P ; Rat. PubMed:27023352 [IF=1.69] GAO, DAN, et al. "Effect of peritoneal dialysis on expression of vascular endothelial growth factor, basic fibroblast growth factor and endostatin of the peritoneum in peritoneal dialysis patients." Nephrology 16.8 (2011): 736-742. WB ; Human. PubMed:21771176 |
|
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 免疫学 生长因子和激素 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 20/190kDa |
细胞定位 | 细胞外基质 分泌型蛋白 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Endostatin:1581-1680/1754 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene. Function: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Present in multiple organs with highest levels in liver, lung and kidney. Post-translational modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. DISEASE: Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Similarity: Belongs to the multiplexin collagen family. Contains 1 FZ (frizzled) domain. Contains 1 TSP N-terminal (TSPN) domain. SWISS: P39060 Gene ID: 1306 Database links: Entrez Gene: 1306 Human Entrez Gene: 80781 Human Entrez Gene: 12819 Mouse Entrez Gene: 12822 Mouse Entrez Gene: 85251 Rat Omim: 120328 Human SwissProt: P39059 Human SwissProt: P39060 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 内皮抑素(endostatin)又称胶原蛋白18A1,是目前较理想的血管形成抑制因子,它通过抑制肿瘤血管生成达到阻止肿瘤生长和转移的目的。当前,血管内皮抑制素主要用于抑止肿瘤血管的生长方面的研究。 |
产品图片 | Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Endostatin (bs-0547R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/190 kD Observed band size: 155 kD Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Endostatin Polyclonal Antibody, Unconjugated(bs-0547R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。