PKM2丙酮酸激酶-M2抗体

价格¥780 - 2200

品牌LMAI Bio
货号LM-0101R
产地进口/国产
更新日期2025年07月11日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,

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供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-0101R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
PKM2
抗体名：
丙酮酸激酶-M2抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
免疫原：
KLH conjugated synthetic peptide derived from human PKM2:255-350/531
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
50ul 100ul 200ul

PKM2丙酮酸激酶-M2抗体

英文名称	PKM2
中文名称	丙酮酸激酶-M2抗体
别名	PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PKM2; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3; KPYM_HUMAN.
	Specific References (2) \| bs-0101R has been referenced in 2 publications. [IF=7.25] Yao, Chun, et al. "Role of FADD Phosphorylation in Regulating Glucose Homeostasis: from Proteomic Discovery to Physiological Validation." Molecular & Cellular Proteomics (2013). WB ; Mouse. PubMed:23828893 [IF=3.15] Li, Hongyan, et al. "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." Toxicology Letters (2014). WB ; Mouse. PubMed:24440814
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	肿瘤免疫学染色质和核信号信号转导细胞周期蛋白激酶和磷酸酶肿瘤细胞生物标志物新陈代谢
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	57kDa
细胞定位	细胞核细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human PKM2:255-350/531
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Subunit: Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. Tissue Specificity: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. ISGylated. Under hypoxia, hydroxylated by EGLN3. Similarity: Belongs to the pyruvate kinase family. SWISS: P14618 Gene ID: 5315 Database links: Entrez Gene: 5315 Human Entrez Gene: 18746 Mouse Entrez Gene: 25630 Rat Omim: 179050 Human SwissProt: P14618 Human SwissProt: P52480 Mouse SwissProt: P11980 Rat Unigene: 534770 Human Unigene: 326167 Mouse Unigene: 405069 Mouse Unigene: 1556 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 丙酮酸激酶（PK）有L、R、M1、M2四种同功酶，均为四聚体。 L型主要分布于肝脏，R型存在于红细胞，在结构、免疫学和动力学上十分相似，由同一基因调控；M1存在于肌肉中，M2分布于除上述以外的其他组织（尤以肾脏最多）以及胎儿各组织。PK也是一种癌胚蛋白，在恶性肿瘤中，增高的都是M2型。
产品图片	Sample:Heart(Mouse) Lysate at 40 ug Primary: Anti- PKM2 (bs-0101R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 54kD Sample:Brain (Mouse) Lysate at 40 ug Primary: Anti- PKM2 (bs-0101R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 54kD Sample:Muscle (Mouse) Lysate at 40 ug Primary: Anti- PKM2 (bs-0101R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 55kD Tissue/cell: human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PKM2 Polyclonal Antibody, Unconjugated(bs-0101R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PKM2 Polyclonal Antibody, Unconjugated(bs-0101R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.