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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
APC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)
- 浓度:
0.2 mg/mL
- 应用范围:
见产品说明书
- 宿主:
Mouse
- 供应商:
嵘崴达
- 保质期:
见产品说明书
- 适应物种:
见产品说明书
- 抗原来源:
见产品说明书
- 库存:
需确认
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 亚型:
IgG2a
- 规格:
100 ug
货号 17-0900-82
100 µg产品描述
Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.
Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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文献和实验CD90 分子 CD90 常用单克隆抗体或代号: 5E10;(Thy1.23) 主要表达细胞: Pre- B,Pro- Hem,大脑[AS] 分子质量(kDa)和结构: gp 25- 35 (GPI连接)(IgSF) 功 能: T细胞活化、识别、粘附 CD90 Summary: May mediate differentiation of hemaoietic stem cells and synaptogenesis
: Monoclonal rat anti‐BrdU antibody (Abcam, cat. no. ab6326) for CldU detection Monoclonal mouse anti‐BrdU antibody (BD, cat. no. 7580) for IdU detection
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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