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发货周期:现货
博士德生物
cell culture supernates, serum and plasma( heparin, EDTA, citrate).
96T
货号 | EK0397 |
---|---|
产品名称 | Human IL-2 ELISA Kit |
产品描述 | For quantitative detection of human IL-2 in cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
免疫原 | E.coli,A21-T153 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | IL2 |
基因全名 | interleukin 2 |
Uniprot ID | P60568 |
指标别名 | Aldesleukin|IL 2|IL-2|IL2|IL2_HUMAN|Interleukin 2|Interleukin-2|Interleukin2|Involved in regulation of T cell clonal expansion|Lymphokine|T cell growth factor|T-cell growth factor|TCGF |
背景资料 | Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0397-CAP | 96-well plate precoated with anti- human IL-2 antibody | 1 |
EK0397-ST | Lyophilized recombinant human IL-2 standard | 10ng/tubex2 |
EK0397-DA | Biotinylated anti- human IL-2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 31 | 158 | 484 | 28 | 169 | 474 |
Standard deviation | 1.27 | 8.84 | 30 | 1.14 | 10.98 | 38.39 |
CV(%) | 4.1% | 5.6% | 6.2% | 4.1% | 6.5% | 8.1% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.005 | 0.049 | 0.106 | 0.199 | 0.371 | 0.734 | 1.471 | 2.213 |
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