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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 保质期:
见产品说明书
- 库存:
需确认
- 保存条件:
见说明书
- CAS号:
见说明书
- 英文名:
Recombinant Pro-TNF-alpha Fusion Protein, CF
- 规格:
10 µg
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文献和实验The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
At present the protein expression systems used commonly by researchers incorporate an affinity tail fused to the protein of interest. These affinity tails provide a convenient and efficient method for the purification of the expressed fusion
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
resuspension buffer and 1 volume thrombin cleavage buffer. Seal the bottom of the column. Add 1 column volume thrombin cleavage buffer plus thrombin. 10 ug per mg fusion protein is a good starting point. Incubate 30-60 minutes at room temp rocking
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