MDR1/P Glycoprotein antibody

价格¥780 - 2200

品牌康朗生物
货号kl-1468R
产地中国/美国/德国
更新日期2025年07月14日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1µg/Test IF=1:100-500
宿主Rabbit
适应物种Human, Mouse, Rat,

上海康朗生物科技有限公司

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供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-1468R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
MDR1/P Glycoprotein antibody
抗体名：
多药耐药蛋白/P-糖蛋白抗体(C端)
宿主：
Rabbit
适应物种：
Human, Mouse, Rat,
免疫原：
KLH conjugated synthetic peptide derived from human MDR1:1051-1280/1280 <Cytoplasmic>
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1µg/Test IF=1:100-500
浓度：
1mg/ml
保存条件：
-20 °C
规格：
50ul 100ul 200ul

MDR1/P Glycoprotein antibody

中文名称	多药耐药蛋白/P-糖蛋白抗体(C端)
别名	ABC20; ABCB1; ATP binding cassette, sub family B (MDR/TAP), member 1; ATP-binding cassette sub-family B member 1; CD243; CLCS; Colchicin sensitivity; Doxorubicin resistance; GP170; MDR1; MDR1_HUMAN; Multidrug resistance 1; Multidrug resistance protein 1; P glycoprotein 1; P gp; P-glycoprotein 1; PGY1. p-gp; P糖蛋白
	Specific References (1) \| bs-1468R has been referenced in 1 publications. [IF=3.23] Mo L, Pospichalova V, Huang Z, Murphy SK, Payne S, Wang F, et al. (2015) Ascites Increases Expression/Function of Multidrug Resistance Proteins in Ovarian Cancer Cells. PLoS ONE 10(7): e0131579. WB ; Human. PubMed:26148191
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	肿瘤细胞生物免疫学信号转导细胞凋亡转录调节因子糖蛋白
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1µg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	141kDa
细胞定位	细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human MDR1:1051-1280/1280 <Cytoplasmic>
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas. Function: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Subunit: Interacts with PSMB5. Subcellular Location: Cell membrane; Multi-pass membrane protein (By similarity). Tissue Specificity: Expressed in liver, kidney, small intestine and brain. DISEASE: Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. Similarity: Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. Gene ID: 5243 Database links: Entrez Gene: 5243 Human Entrez Gene: 18669 Mouse Entrez Gene: 170913 Rat Entrez Gene: 24646 Rat Omim: 171050 Human SwissProt: P08183 Human SwissProt: P06795 Mouse SwissProt: P43245 Rat Unigene: 489033 Human Unigene: 144554 Rat Unigene: 154810 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 信号传导（Signaling Intermediates）
产品图片	Sample: liver (Rat) Lysate at 40 ug Primary: Anti-MDR1'P Glycoprotein (bs-1468R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 141kD Observed band size: 141 kD Tissue/cell: Rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MDR1 / P Glycoprotein Polyclonal Antibody, Unconjugated(bs-1468R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (bs-1468R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.