Uricase 尿酸酶-10828475103 from Arthrobacter protophormiae, lyophilizate
Oxidase that catalyzes the oxidation of uric acid to 5-hydroxyisourate which
decomposes to allantoin under in vitro conditions.
Application Use Uricase in diagnostic tests for the determination of uric acid and for the
elimination of uric acid interferences.
Benefits Eliminate uric acid interferences. ■
Rely on the proven diagnostic quality. ■
EC 1.7.3.3
Properties Nomenclature: Urate:oxygen oxidoreductase
Molecular weight: ~170 kD, with four subunits of ~40 kD
Michaelis constant (Phosphate buffer, 0.1 mol/l, pH 8.0; +25°C):
Urate: 6.6 x 10 -5 mol/l
Stabilizer/activators: EDTA is good for stabilization. DTT or DTE may show
a stabilizing effect depending on reagent composition. Triton X-100 (1-2 ml/l)
may show an activating effect.
Inhibitors: Zn 2+ , Cl - (Tris-HCI buffer is not suitable) and borate inhibit strongly.
NaN 3 , 0.1% does not inhibit.
pH optimum: 9.0 (see figure). Roche uric acid reagent contains phosphate
buffer, pH 7.8. For these conditions, high activity and higher stability of the
Uricase are achieved.
Temperature dependence: See figure, above +50°C there is a decrease in
activity due to lower O 2 concentration.
pH stability: 6.5-10.0 (see figure)
Thermal stability: +20°C to +60°C (see figure)
Specificity: Uricase is specific for urea.
Specification Appearance: White lyophilizate
pH value (c=10 mg/ml in water): 6.7-7.5
Protein (Biuret): No limit
Activity (+25°C, urate, saturated with O 2 , pH 8.5): ≥20 U/mg lyophilizate
Specific activity: ≥50 U/mg protein
Contaminant (expressed as percentage of Uricase activity):
Catalase: ≤0.5
Stability: At +2 to +8°C within specification range for 12 months. Store dry.