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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 库存:
需确认
- 克隆性:
单克隆
- 保质期:
见产品外包装
- 抗体英文名:
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
- 亚型:
IgG1
- 保存条件:
2-8°C
- 规格:
1 vial
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
clone RG-96, purified from hybridoma cell culture
别名:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
一般描述
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
免疫原
Rabbit IgG
应用
Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
- various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
- westernblotting
- immunohistology
- dot immunobinding assay
外形
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
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