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- 4634772001
- 2025年10月22日
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Genopure Buffer Set
Genopure Buffer Set
4634772001
一般描述
Doubling the volume of the Suspension, Lysis, and Neutralization Buffers during the Genopure plasmid preparation procedure will provide better yields of plasmid DNA from low-copy number plasmids. The buffers in this set may be used with either of the Genopure Plasmid Kits (Midi or Maxi).
应用
Doubling the volume of the Suspension, Lysis, and Neutralization Buffers during the Genopure plasmid preparation procedure will provide better yields of plasmid DNA from low-copy number plasmids. The buffers in this set may be used with either of the Genopure Plasmid Kits (Midi or Maxi).
特点和优势
The Genopure Buffer Set for Low-Copy Number Plasmids provides additional buffers to supplement those in the Genopure Plasmid Kits.
RNase A is also included in the set because it eliminates bacterial RNA from the preparation, thereby improving the recovery of low copy number plasmids.
- Convenient, ready-to-use, function-tested, nuclease-free buffers
- Helps the Genopure Plasmid Midi and Maxi Kits enhance plasmid yield
- Ensures reproducible results
组分
- Suspension Buffer, 500 mL
- RNase A, 50 mg
- Lysis Buffer, 500 mL
- Neutralization Buffer, 500 mL
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
no special instrumentation is necessary, and can be used to validate the quality of protein preparations, screen for ligands or cofactors, and discover buffers and additives that maximize protein stability. This unit describes how to set up a Thermofluor
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