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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-13526R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
GPR25 antibody
- 抗体名:
G蛋白偶联受体25抗体
- 宿主:
Rabbit
- 适应物种:
Human
- 免疫原:
KLH conjugated synthetic peptide derived from human G protein coupled receptor 25:81-180/361 <Extracellular>
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul 200ul
| 中文名称 | G蛋白偶联受体25抗体 |
| 别 名 | G protein coupled receptor 25; Gpr25; GPR25_HUMAN; Probable G protein coupled receptor GPR25; Probable G-protein coupled receptor 25; GPCR GPR25. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 心血管 细胞生物 信号转导 生长因子和激素 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 25:81-180/361 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein-coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR25 (G protein-coupled receptor 25) is a 361 amino acid multi-pass membrane protein that belongs to the G protein-coupled receptor family and functions as an orphan receptor, possibly playing a role in signal transduction throughout the cell. The gene encoding GPR25 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Function: Orphan receptor. Subcellular Location: Cell membrane. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: O00155 Gene ID: 2848 Database links: Entrez Gene: 2848 Human Omim: 602174 Human SwissProt: O00155 Human Unigene: 534316 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR25 Polyclonal Antibody, Unconjugated(bs-13526R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining ![]() Tissue/cell: human meningioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR25 Polyclonal Antibody, Unconjugated(bs-13526R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining ![]() Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR25 Polyclonal Antibody, Unconjugated(bs-13526R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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