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- 供应商:
原生原代
- 库存:
大量
- 英文名:
Penicillin–Streptomycin–Amphotericin B Solution
- 规格:
1ml
Penicillin–Streptomycin–Amphotericin B Solution
产品编号:AG-0005
产品名称:Penicillin–Streptomycin–Amphotericin B Solution for Primary Cells
产品规格:1ml
产品价格:¥198.00
-20°C储存;冷藏运输
不含HIV、HBV、HCV、细菌、支原体、真菌、酵母;不含有内毒素;不含有苯;不含有血清;
生物安全1级。
只可用于科研。
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文献和实验b-galactosidase Solution Assay
.1 g up to 500 ml 50 mM CPRG 0.0304 g chlorophenyl red b-galactopyranoside (Boehringer Mannheim #834-308) up to 1 ml with Q store in the dark at -20° Procedure • Wash cells or tissue twice in PBS, pellet and resuspend in 150 microliters
b-galactosidase Solution Assay
b-galactosidase Solution Assay Reference: Simon and Lis (1987) NAR 15 : 2971-2988. Solutions CPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl 2 up to 100 ml with Q
Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
Primary murine splenic B cells can be cultured ex vivo and, following treatment with LPS, cytokines and/or CD40L proliferate, and undergo class switch recombination and terminal differentiation to become immunoglobulin-secreting plasmacytic cells
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