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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant Rat PDGF-BB
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
2μg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM6389-2μg | Recombinant Rat PDGF-BB | 2μg | 464.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Rat | 24628 | LM05028 | E. coli | 109aa | 24.4kDa | - |
| About this Antibody | |
| Name | Recombinant Rat PDGF-BB (Recombinant Rat Platelet-derived Growth Factor-BB; rRtPDGF-BB);重组大鼠血小板衍生生长因子-BB |
| Synonyms | Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Pdgfb |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0ng/ml, corresponding to a specific activity of >5.0×105IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH6.9. |
| Endotoxin | Less than 0.1EU/μg of rRtPDGF-BB as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGFA and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant rat PDGF-AA contains 109 amino acids and has a molecular mass of 12.2kDa. |
| Amino Acid Sequence | SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT |
| 产品编号 | 产品名称 | 包装 |
| LM6389-2μg | Recombinant Rat PDGF-BB | 2μg |
| LM6389-10μg | Recombinant Rat PDGF-BB | 10μg |
| LM6389-100μg | Recombinant Rat PDGF-BB | 100μg |
| LM6389-1mg | Recombinant Rat PDGF-BB | 1mg |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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