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Sigma货号B5002-1g现货5-溴-2′-脱氧尿苷[5

-Bromo-2′-deoxyuridine]上海睿安生物19121878899
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  • ￥1775.31
  • Sigma-Aldrich
  • B5002-1g
  • made in USA
  • 2026年01月01日
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    上海睿安生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存：

      100⁺瓶

    • 英文名：

      5-Bromo-2′-deoxyuridine

    • CAS号：

      59-14-3

    • 保质期：

      1年

    • 供应商：

      上海睿安生物13611631389

    • 保存条件：

      -20°C

    • 规格：

      1G

    Sigma货号B5002-1g现货5-溴-2′-脱氧尿苷[5-Bromo-2′-deoxyuridine]上海睿安生物19121878899

    5-溴-2′-脱氧尿苷

    ≥99%(HPLC),synthetic(organic),powder

    别名:5-BrdU，5-溴-1-(2-脱氧-β-D-呋喃核糖)尿嘧啶，5-溴脱氧尿苷，BUdR

    说明

    应用

    5-溴-2′-脱氧尿苷用于:
    • 用作研究电痉挛性癫痫对海马神经发生的影响的补充剂
    • 用作研究斑马鱼成鱼基因丧失功能的补充剂
    • 心脏T淋巴细胞增殖试验{133]
    5-溴-2′-脱氧尿苷(5-BrdU)是一种可插入到DNA中的胸苷类似物。5-BrdU日常被广泛用于对DNA合成进行测量以及对分裂的细胞进行标记。因此，5-BrdU用于研究细胞信号传导和诱导细胞增殖的其他过程。

    生化/生理作用

    5-溴-2′-脱氧尿苷(BrdU)用于分析细胞增殖，因为它在细胞周期的S期可轻松掺入DNA。BrdU刺激白血病细胞系中的细胞分化和成熟，同时抑制Friend红白血病细胞分化。随后通过诸如流式细胞分析或荧光显微分析方法研究掺入BrdU时，通常使用带有荧光标记的BrdU特异性抗体来检测掺入的BrdU。
    用作遗传研究中诱变剂的胸苷类似物。在S期可选择性结合到细胞DNA中。
    产品细节图片1

    常见问题

    1. How does BrdU, Product B5002, work as a Thymidine analog?

      BrdU replaces Thymidine during the S-phase of cell division.

    2. What are the hazards associated with BrdU, Product B5002?

      BrdU is a known carcinogen and caution should be exercised when handling.  Click on the MSDS hyperlink located on the product detail page for Product No. B5002, 5-Bromo-2´-deoxyuridine. 

    3. How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?

      Actively growing cells may be treated with 10 μm BrdU for 1 hour.

    4. What can I use to detect BrdU, Product B5002 in vivo and in vitro?

      For the immunohistochemical detection of BrdU, we recommend Product No. B2531, Monoclonal Anti-BrdU antibody produced in mouse.

    5. What is the stability of Product B5002, BrdU?

      BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002.  Please consult our Product Dating Information Statement for more information on re-test dated products.

    6. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    7. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

     

    同行评审论文(部分文献)

     
    Target Validation in Drug Discovery,109-109(2011)
     
    Mechanisms of Differentiation, Volume 2, 85-85 (1990)
    Electroconvulsive seizures increase hippocampal neurogenesis after chronic corticosterone treatment
    Hellsten J, et al.
    The European Journal of Neuroscience, 16(2), 283-290 (2002)
    PEG-PLA nanoparticles facilitate siRNA knockdown in adult zebrafish heart
    Diao J, et al.
    Developmental Biology, 406, 196-202 (2015)
    Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice.
    Francisco Alén et al.
    International journal of molecular sciences, 20(14) (2019-07-25)
    Mutant mice with respect to the splicing factor Zrsr1 present altered spermatogenesis and infertility. To investigate whether Zrsr1 is involved in the homeostatic control that the hypothalamus exerts over reproductive functions, we first analyzed both differential gene and isoform expression
     
    产品细节图片2产品细节图片3产品细节图片4产品细节图片5产品细节图片6产品细节图片7产品细节图片8产品细节图片9

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    图标文献和实验
    该产品被引用文献

    Sigma货号B5002-1g现货5-溴-2′-脱氧尿苷[5-Bromo-2′-deoxyuridine]上海睿安生物19121878899

    5-溴-2′-脱氧尿苷

    ≥99%(HPLC),synthetic(organic),powder

    别名:5-BrdU，5-溴-1-(2-脱氧-β-D-呋喃核糖)尿嘧啶，5-溴脱氧尿苷，BUdR

    说明

    应用

    5-溴-2′-脱氧尿苷用于:
    • 用作研究电痉挛性癫痫对海马神经发生的影响的补充剂
    • 用作研究斑马鱼成鱼基因丧失功能的补充剂
    • 心脏T淋巴细胞增殖试验{133]
    5-溴-2′-脱氧尿苷(5-BrdU)是一种可插入到DNA中的胸苷类似物。5-BrdU日常被广泛用于对DNA合成进行测量以及对分裂的细胞进行标记。因此，5-BrdU用于研究细胞信号传导和诱导细胞增殖的其他过程。

    生化/生理作用

    5-溴-2′-脱氧尿苷(BrdU)用于分析细胞增殖，因为它在细胞周期的S期可轻松掺入DNA。BrdU刺激白血病细胞系中的细胞分化和成熟，同时抑制Friend红白血病细胞分化。随后通过诸如流式细胞分析或荧光显微分析方法研究掺入BrdU时，通常使用带有荧光标记的BrdU特异性抗体来检测掺入的BrdU。
    用作遗传研究中诱变剂的胸苷类似物。在S期可选择性结合到细胞DNA中。
    引用文献图片1

    常见问题

    1. How does BrdU, Product B5002, work as a Thymidine analog?

      BrdU replaces Thymidine during the S-phase of cell division.

    2. What are the hazards associated with BrdU, Product B5002?

      BrdU is a known carcinogen and caution should be exercised when handling.  Click on the MSDS hyperlink located on the product detail page for Product No. B5002, 5-Bromo-2´-deoxyuridine. 

    3. How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?

      Actively growing cells may be treated with 10 μm BrdU for 1 hour.

    4. What can I use to detect BrdU, Product B5002 in vivo and in vitro?

      For the immunohistochemical detection of BrdU, we recommend Product No. B2531, Monoclonal Anti-BrdU antibody produced in mouse.

    5. What is the stability of Product B5002, BrdU?

      BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002.  Please consult our Product Dating Information Statement for more information on re-test dated products.

    6. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    7. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

     

    同行评审论文(部分文献)

     
    Target Validation in Drug Discovery,109-109(2011)
     
    Mechanisms of Differentiation, Volume 2, 85-85 (1990)
    Electroconvulsive seizures increase hippocampal neurogenesis after chronic corticosterone treatment
    Hellsten J, et al.
    The European Journal of Neuroscience, 16(2), 283-290 (2002)
    PEG-PLA nanoparticles facilitate siRNA knockdown in adult zebrafish heart
    Diao J, et al.
    Developmental Biology, 406, 196-202 (2015)
    Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice.
    Francisco Alén et al.
    International journal of molecular sciences, 20(14) (2019-07-25)
    Mutant mice with respect to the splicing factor Zrsr1 present altered spermatogenesis and infertility. To investigate whether Zrsr1 is involved in the homeostatic control that the hypothalamus exerts over reproductive functions, we first analyzed both differential gene and isoform expression
     
    引用文献图片2引用文献图片3引用文献图片4引用文献图片5引用文献图片6引用文献图片7引用文献图片8引用文献图片9
    相关实验

    • 常用生化试剂

      Amresco分装 B1053  5-Bromo-2'-Deoxyuridine(5-溴-2-脱氧尿苷) 250mg 450 Fluka分装 B1054  BSA,Fraction V  (牛血清白蛋白) 5g/25g/100g 70/250/700

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    文献支持
    Sigma货号B5002-1g现货5-溴-2′-脱氧尿苷[5-Bromo-2′-deoxyuridine]上海睿安生物19121878899
    ￥1775.31