- ￥2200.01
- Proteintech
- 10380-1-AP
- made in China
- 2025年12月27日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Caspase 9/P35 Polyclonal antibody
- 保质期：
1年
- 保存条件：
-20°C
- 库存：
10⁺支
- 供应商：
上海睿安生物19121878899
- 规格：
100μl/支
特惠促销Proteintech货号10380-1-AP现货Caspase 9/P35 Polyclonal antibody上海睿安生物19121878899
Caspase 9/P35 Polyclonal antibody
Caspase 9/P35 Polyclonal Antibody for WB、IHC、IF/ICC、IP、ELISA
经过测试的应用
Positive WB detected in Jurkat cells, Staurosporine treated Jurkat cells
Positive IP detected in HeLa cells
Positive IHC detected in mouse lung tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in HepG2 cells
上
海
推荐稀释比
应用 推荐稀释比
Western Blot (WB) WB : 1:300-1:1000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:50-1:500
Immunofluorescence (IF)/ICC IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
背景介绍
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
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文献和实验特惠促销Proteintech货号10380-1-AP现货Caspase 9/P35 Polyclonal antibody上海睿安生物19121878899
Caspase 9/P35 Polyclonal antibody
Caspase 9/P35 Polyclonal Antibody for WB、IHC、IF/ICC、IP、ELISA
经过测试的应用
Positive WB detected in Jurkat cells, Staurosporine treated Jurkat cells
Positive IP detected in HeLa cells
Positive IHC detected in mouse lung tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in HepG2 cells
上
海
推荐稀释比
应用 推荐稀释比
Western Blot (WB) WB : 1:300-1:1000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:50-1:500
Immunofluorescence (IF)/ICC IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
背景介绍
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
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