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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Hin1II (NlaIII) (5 U/µL)
- 保质期：
1年
- 供应商：
上海沪震实业有限公司
- 保存条件：
2-8℃保存
- 规格：
300units
Hin1II (NlaIII) (5 U/µL)
Enzyme : Hin1II (NlaIII)保存温度 : -20℃
货期 : 2-3天
Compatible Buffer : 10x Buffer G
Optimal Reaction Temperature : 37° C
Sensitive to Heat Inactivation: : Yes
Methylation Sensitivity : Not CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive
5' C A T G ↓ 3'
3' ↑ G T A C 5'
Thermo Scientific Hin1II (NlaIII) restriction enzyme recognizes CATG^ sites and cuts best at 37°C in G buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
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文献和实验Characterization of Angiotensin II Receptors
) 80 µM unlabeled Ang II working solution (see recipe ) Unlabeled peptides and test compounds in 8% DMSO
x PCR reaction buffer, 1.6 µl 4dNTP mix (250 pmol/µl), 2 µl T12 MN primer, 2 µl arbitrary primer (decamer), 0.2 µl Taq DNA polymerase (5 U/µl) use the PCR conditions as under section III. run PCR product on a 1.5 % agarose gel
Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
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