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DEAE (diethylaminoethyl) phase30 μm
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文献和实验Antibiotic Concentration in Media
Chlroramphenicol 500 (C) 30 (C/P) 170 µg/ml for plasmid amplification 34 mg/ml in EtOH: -20 C D-cycloserine 200 (C) Add dry after autoclaving Erythromycin 200 (C) 25 mg/ml EtOH: -20 C Gentamycin 10 (C/P) Add dry after autoclaving Geneticin 200 for fungi
with release from anthers but is not essential), then dab anthers onto surface of a 25-30µL drop of germination media. Drop can be on a depression slide or on a coated slide designed to keep samples separate. Use 1 flower per drop. Invert and incubate overnight
Yeast Media, Solutions and Stocks
autoclave 30-45 minutes and cool to 65 degrees C, then add 50 ml of sterile 40% glucose YPD plates Add 20 g Bacto agar before autoclaving Synthetic Media Supplements All stock solutions
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