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- EASYBIO
- BE7007-100
- 2026年04月17日
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MBP-HRP Mouse Monoclonal Antibody
Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP Tag antibody is suitable for detecting fusion proteins that contain a MBP-tag.
Product Name: MBP-tag mouse monoclonal antibody- HRP conjugated
Clone Number: 8G3
Isotype: IgG1
Immunogen: Full-length MBP protein
Tested applications: WB. Not yet tested in other applications.
Recommended dilutions: WB: 1:2,000-5,000
Optimal dilutions/concentrations should be determined by the end user.
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: MBP antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Western blot analysis of MBP-tag fusion protein with MBP-tag mouse mAb(8G3) diluted at 1:2000.
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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